Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 4053021..4053618 Replicon chromosome
Accession NZ_CP067261
Organism Klebsiella variicola strain E25

Toxin (Protein)


Gene name higB Uniprot ID A0A0B7GEF2
Locus tag JJB14_RS19870 Protein ID WP_012542526.1
Coordinates 4053301..4053618 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag JJB14_RS19865 Protein ID WP_012542525.1
Coordinates 4053021..4053308 (-) Length 96 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJB14_RS19835 (JJB14_19830) 4048931..4049179 + 249 WP_008805539.1 DUF1158 domain-containing protein -
JJB14_RS19840 (JJB14_19835) 4049196..4049537 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
JJB14_RS19845 (JJB14_19840) 4049568..4050683 - 1116 WP_162493283.1 MBL fold metallo-hydrolase -
JJB14_RS19850 (JJB14_19845) 4050863..4051444 + 582 WP_012968754.1 TetR/AcrR family transcriptional regulator -
JJB14_RS19855 (JJB14_19850) 4051444..4051812 + 369 WP_008805536.1 MmcQ/YjbR family DNA-binding protein -
JJB14_RS19860 (JJB14_19855) 4051932..4052585 + 654 WP_012968755.1 oxygen-insensitive NAD(P)H nitroreductase -
JJB14_RS19865 (JJB14_19860) 4053021..4053308 - 288 WP_012542525.1 helix-turn-helix transcriptional regulator Antitoxin
JJB14_RS19870 (JJB14_19865) 4053301..4053618 - 318 WP_012542526.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JJB14_RS19875 (JJB14_19870) 4053803..4054846 - 1044 WP_016160437.1 DUF2157 domain-containing protein -
JJB14_RS19880 (JJB14_19875) 4055376..4056242 - 867 WP_208777590.1 helix-turn-helix transcriptional regulator -
JJB14_RS19885 (JJB14_19880) 4056351..4057778 + 1428 WP_023297179.1 MFS transporter -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(12-100)

Antitoxin

(38-89)


Sequences


Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T185970 WP_012542526.1 NZ_CP067261:c4053618-4053301 [Klebsiella variicola]
MFRLVVHVDVKKELQALPPIVQAKMIRQIDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T185970 NZ_CP089097:847562-847972 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10815.51 Da        Isoelectric Point: 10.2212

>AT185970 WP_012542525.1 NZ_CP067261:c4053308-4053021 [Klebsiella variicola]
MSKKIIDWDTLKTELLSQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQILLY

Download         Length: 288 bp

>AT185970 NZ_CP089097:847315-847581 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTATGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A0B7GEF2


Antitoxin

Source ID Structure

References