Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 352766..353412 Replicon chromosome
Accession NZ_CP067261
Organism Klebsiella variicola strain E25

Toxin (Protein)


Gene name higB Uniprot ID A0A231WWF9
Locus tag JJB14_RS01600 Protein ID WP_004174016.1
Coordinates 352766..353113 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A1F2LZT5
Locus tag JJB14_RS01605 Protein ID WP_008806992.1
Coordinates 353113..353412 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJB14_RS01590 (JJB14_01590) 348653..350086 + 1434 WP_004202133.1 glycogen synthase GlgA -
JJB14_RS01595 (JJB14_01595) 350104..352551 + 2448 WP_008806990.1 glycogen phosphorylase -
JJB14_RS01600 (JJB14_01600) 352766..353113 + 348 WP_004174016.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JJB14_RS01605 (JJB14_01605) 353113..353412 + 300 WP_008806992.1 XRE family transcriptional regulator Antitoxin
JJB14_RS01610 (JJB14_01610) 353475..354983 - 1509 WP_004202136.1 glycerol-3-phosphate dehydrogenase -
JJB14_RS01615 (JJB14_01615) 355188..355517 + 330 WP_032733885.1 thiosulfate sulfurtransferase GlpE -
JJB14_RS01620 (JJB14_01620) 355568..356398 + 831 WP_032740277.1 rhomboid family intramembrane serine protease GlpG -
JJB14_RS01625 (JJB14_01625) 356448..357206 + 759 WP_008806995.1 DeoR/GlpR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(31-84)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13518.55 Da        Isoelectric Point: 6.2327

>T185957 WP_004174016.1 NZ_CP067261:352766-353113 [Klebsiella variicola]
MWDVETTDTFDAWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGNKDGMNEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

>T185957 NZ_CP089093:18965-19222 [Enterococcus faecium]
ATGATTAAGGCTTGGTCTGATGATGCTTGGGATGATTATCTTTATTGGCATGAGCAAGGAAACAAAAGCAATATAAAAAA
GATTAACAAGTTAATAAAAGATATCGATCGTTCCCCCTTTGCTGGATTAGGAAAACCTGAGCCATTAAAGCATGATTTAT
CTGGAAAATGGTCCAGAAGAATTACAGATGAACATAGACTGATATATAGAGTTGAAAATGAAACGATATTTATTTATTCT
GCAAAAGATCACTATTAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10814.61 Da        Isoelectric Point: 10.1464

>AT185957 WP_008806992.1 NZ_CP067261:353113-353412 [Klebsiella variicola]
MATLKELMAKQSPESQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT185957 NZ_CP089093:18703-18972 [Enterococcus faecium]
ATGGAAGCAGTAGCTTATTCAAATTTCCGCCAAAATTTACGTAGTTATATGAAACAAGTTAATGAGGATGCTGAAACACT
TATTGTAACAAGTAAAGATGTAGAAGATACAGTTGTTGTATTATCAAAAAGAGATTATGATTCTATGCAAGAAACGTTGA
GAACACTTTCTAATAATTACGTCATGGAAAAAATTCGTCGAGGAGATGAACAATTCTCCAAAGGTGCATTTAAAACACAT
GACTTAATCGAGGTTGAATCTGATGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A231WWF9

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2LZT5

References