Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 349413..350059 Replicon chromosome
Accession NZ_CP067255
Organism Klebsiella pneumoniae strain ESBL_DR09

Toxin (Protein)


Gene name higB Uniprot ID W9BBY1
Locus tag JJB15_RS01615 Protein ID WP_016529833.1
Coordinates 349413..349760 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID W8UB68
Locus tag JJB15_RS01620 Protein ID WP_002920557.1
Coordinates 349760..350059 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJB15_RS01605 (345349) 345349..346782 + 1434 WP_002920564.1 glycogen synthase GlgA -
JJB15_RS01610 (346800) 346800..349247 + 2448 WP_002920561.1 glycogen phosphorylase -
JJB15_RS01615 (349413) 349413..349760 + 348 WP_016529833.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JJB15_RS01620 (349760) 349760..350059 + 300 WP_002920557.1 helix-turn-helix transcriptional regulator Antitoxin
JJB15_RS01625 (350122) 350122..351630 - 1509 WP_002920554.1 glycerol-3-phosphate dehydrogenase -
JJB15_RS01630 (351835) 351835..352164 + 330 WP_002920552.1 thiosulfate sulfurtransferase GlpE -
JJB15_RS01635 (352215) 352215..353045 + 831 WP_004151408.1 rhomboid family intramembrane serine protease GlpG -
JJB15_RS01640 (353095) 353095..353853 + 759 WP_002920548.1 DeoR/GlpR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

HigA2-like, helix-turn-helix domain

(9-87)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13549.56 Da        Isoelectric Point: 5.6749

>T185935 WP_016529833.1 NZ_CP067255:349413-349760 [Klebsiella pneumoniae]
MWDVETTDTFDTWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGDKDGMNEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

>T185935 NZ_CP089065:c4978238-4977987 [Pseudomonas aeruginosa]
ATGCGCACGATCAGTTACACCGAAGCCCGGCAGAACCTGTCGGCCACCATGATGAAGACCGTGGAGGATCGCGCCCCGAT
CCTGATTACCCGGCAGAACGGCGAAGCCTGCGTGTTGATGTCCCTGGAAGAGTTCAACGCGCTGGAGGAAACGGCTTATC
TGCTGCGCTCCCCGGCCAATGCCCGCCGCCTGATGGATTCGATTGACAGCCTCAAGCAAGGCCAGGGCGTCGAGCGGGAT
TTGGCCGAGTGA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10800.58 Da        Isoelectric Point: 10.1464

>AT185935 WP_002920557.1 NZ_CP067255:349760-350059 [Klebsiella pneumoniae]
MATLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT185935 NZ_CP089065:c4977990-4977736 [Pseudomonas aeruginosa]
GTGAAGCTGATCTGGTCGGAACAAGCTTGGGACGATTATCTGTACTGGCAGGACACGGATAAGCGTATGGTGAAAAAGGT
CAACGAGTTGATCAAGGATGCCTGCCGTACCCCGTTCGAGGGCAAGGGAAAGCCGGAGCCGCTTCGCCACAACCTGGCGG
GCTTCTGGTCGAGACGGATCACGGCCGAGCATCGGTTTGTGTATTCGGTGACGGATGATGCGCTGCTGATTGCCGCCTGC
CGTTACCACTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB W9BBY1

Antitoxin

Source ID Structure
AlphaFold DB A0A0E1CBF8

References