Detailed information of TA system TA185924

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 51747..52001
• Replicon: plasmid pESBL_DR28a
• Accession: NZ_CP067251
• Organism: Escherichia coli strain ESBL_DR28

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: JJB16_RS23210
• Protein ID: WP_001312851.1
• Coordinates: 51747..51896 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	51940..52001 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJB16_RS23180 (46994)	46994..47905	-	912	WP_000440183.1	carbamate kinase	-
JJB16_RS23185 (47916)	47916..49136	-	1221	WP_000410951.1	arginine deiminase	-
JJB16_RS23190 (49843)	49843..50457	+	615	Protein_64	VENN motif pre-toxin domain-containing protein	-
JJB16_RS23195 (50457)	50457..50903	-	447	Protein_65	plasmid replication initiator RepA	-
JJB16_RS23200 (50896)	50896..50970	-	75	WP_032336874.1	RepA leader peptide Tap	-
JJB16_RS23205 (51206)	51206..51463	-	258	WP_000083833.1	replication regulatory protein RepA	-
JJB16_RS23210 (51747)	51747..51896	-	150	WP_001312851.1	Hok/Gef family protein	Toxin
- (51940)	51940..52001	+	62	NuclAT_2	-	Antitoxin
- (51940)	51940..52001	+	62	NuclAT_2	-	Antitoxin
- (51940)	51940..52001	+	62	NuclAT_2	-	Antitoxin
- (51940)	51940..52001	+	62	NuclAT_2	-	Antitoxin
JJB16_RS23215 (52140)	52140..52322	-	183	WP_000968309.1	hypothetical protein	-
JJB16_RS23220 (52423)	52423..53039	+	617	Protein_70	IS1-like element IS1A family transposase	-
JJB16_RS23225 (53077)	53077..54648	-	1572	WP_023149734.1	IS66-like element ISCro1 family transposase	-
JJB16_RS23230 (54668)	54668..55015	-	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
JJB16_RS23235 (55015)	55015..55692	-	678	WP_001339397.1	IS66-like element accessory protein TnpA	-
JJB16_RS23240 (55747)	55747..55836	+	90	Protein_74	IS1 family transposase	-
JJB16_RS23245 (56137)	56137..56349	-	213	WP_005012601.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul1 / qacE / aadA5 / mph(A) / erm(B) / aac(3)-IId / fosA3 / blaCTX-M-65 / sitABCD	iutA / iucD / iucC / iucB / iucA	1..133432	133432

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T185924 WP_001312851.1 NZ_CP067251:c51896-51747 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T185924 NZ_CP089064:26641-27219 [Pseudomonas aeruginosa]
ATGTCCCTCGAACTGCTGAGCAACCGCCTCGAATTCCTCCGCGAAGCCGAACGCCTGAAAGACGTCCTGCGCTCCGCGCA
CACCTCCTCCGGCCGCCCGGAAAGCACCGCCGAACATAGCTGGCGGCTGTGCCTGATGGCACTGGCCTTCGAGGACCAAC
TGGCCGGCCTGGACCTCGGCAAGGTCCTGCGCATGTGCGTGGTCCACGACCTCGGCGAAGCGATCCACGGCGACATCCCC
GCCGTCGAGCAGGCCGCGCACCCGGACAAAGGCGAACAGGAACGCGCCGACCTGTTGCAGCTGACCCGTCACCTCGACGC
TCCGCTGCGCGACCGCCTGCTCGCCCTGTGGGACGAATACGAGCGCGGCGAGTCCGCCGAAGCCCTGGCAGTGAAGGCCC
TGGACAAGCTGGAAACCCTGCTCCAGCACACCCAGGGCGATAATCCGGCGGACTTCGACTATGCCTTCAACCTCGACTAC
GGCCGTCGCTATACCGACCGCGCACCGCTGTTCAAGACCCTGCGCGAGCTACTCGACGCCAGGACCCGACAGCGCCTCGC
AGCTCGGCGCGACGCCTAG

Antitoxin

Nucleotide

• Length: 62 bp

>AT185924 NZ_CP067251:51940-52001 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin