Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3971884..3972683 Replicon chromosome
Accession NZ_CP067250
Organism Escherichia coli strain ESBL_DR28

Toxin (Protein)


Gene name yhaV Uniprot ID D3GWU5
Locus tag JJB16_RS18865 Protein ID WP_000347272.1
Coordinates 3972219..3972683 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag JJB16_RS18860 Protein ID WP_001307405.1
Coordinates 3971884..3972219 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJB16_RS18845 (3967669) 3967669..3968439 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
JJB16_RS18850 (3968455) 3968455..3969789 - 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
JJB16_RS18855 (3970164) 3970164..3971735 + 1572 WP_001273738.1 galactarate dehydratase -
JJB16_RS18860 (3971884) 3971884..3972219 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
JJB16_RS18865 (3972219) 3972219..3972683 + 465 WP_000347272.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
JJB16_RS18870 (3972738) 3972738..3973547 - 810 WP_000072171.1 aga operon transcriptional regulator AgaR -
JJB16_RS18875 (3973796) 3973796..3975076 + 1281 WP_001521382.1 tagatose-bisphosphate aldolase subunit KbaZ -
JJB16_RS18880 (3975099) 3975099..3975572 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
JJB16_RS18885 (3975583) 3975583..3976362 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
JJB16_RS18890 (3976352) 3976352..3977230 + 879 WP_001298314.1 PTS N-acetylgalactosamine transporter subunit IID -
JJB16_RS18895 (3977248) 3977248..3977682 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3962959..3972683 9724


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17832.26 Da        Isoelectric Point: 9.6924

>T185923 WP_000347272.1 NZ_CP067250:3972219-3972683 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEPH

Download         Length: 465 bp

>T185923 NZ_CP089063:6574914-6575195 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCTCTGAAAGCCGTTCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTTAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT185923 WP_001307405.1 NZ_CP067250:3971884-3972219 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT185923 NZ_CP089063:6574690-6574917 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCCTACCACCTGCGGCAGGCGCTCGCGCAATACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829KUD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References