Detailed information of TA system TA185901

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 107670..107923
• Replicon: plasmid pOW1E2a
• Accession: NZ_CP067246
• Organism: Escherichia coli strain OW1E2

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: JJB17_RS23775
• Protein ID: WP_001312851.1
• Coordinates: 107774..107923 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	107670..107729 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJB17_RS23750 (104397)	104397..105143	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
JJB17_RS23755 (105198)	105198..105758	+	561	WP_000139328.1	fertility inhibition protein FinO	-
JJB17_RS23760 (105890)	105890..106090	+	201	WP_015059022.1	hypothetical protein	-
JJB17_RS23765 (106476)	106476..107075	+	600	WP_032083981.1	PIN domain-containing protein	-
JJB17_RS23770 (107137)	107137..107469	+	333	WP_152916585.1	hypothetical protein	-
- (107670)	107670..107729	-	60	NuclAT_1	-	Antitoxin
- (107670)	107670..107729	-	60	NuclAT_1	-	Antitoxin
- (107670)	107670..107729	-	60	NuclAT_1	-	Antitoxin
- (107670)	107670..107729	-	60	NuclAT_1	-	Antitoxin
JJB17_RS23775 (107774)	107774..107923	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
JJB17_RS23780 (108207)	108207..108455	+	249	WP_000083837.1	replication regulatory protein RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	floR / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / fosA3 / blaTEM-1B / blaCTX-M-55	-	1..108766	108766

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T185901 WP_001312851.1 NZ_CP067246:107774-107923 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T185901 NZ_CP089053:50473-50829 [Acinetobacter ursingii]
ATGTGGACAGTCATTACGACAGATCTATTTAATCAGTGGCTTGAACAGCAAGATGAACCTACACAAGAGAAGGTCCTAGC
TGCCCTGGTTGTTCTACAGCAGCAGGGACCGAGTTTAGGCCGTCCTTTAGTAGATACTGTGTATGAGTCTAAATTTACCA
ATATGAAAGAACTGCGTGTTCAACATCGCGGTAGACCCTTAAGAGCTTTCTTCGCATTTGATCCCTTACGACAGGCTATT
GTTCTATGTATTGCTGATAAAGGCGGTAAAAAGCGTTTTTATAAAGACATGCTGGATATTGCAGATGAACAATACCAACT
TCATCTCACTACCCTAGGAGATAAATCTAATGGCTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT185901 NZ_CP067246:c107729-107670 [Escherichia coli]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin