Detailed information of TA system TA185898

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 68210..68479
• Replicon: plasmid pOW1E2a
• Accession: NZ_CP067246
• Organism: Escherichia coli strain OW1E2

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: JJB17_RS23545
• Protein ID: WP_001372321.1
• Coordinates: 68354..68479 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	68210..68275 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJB17_RS23520	63920..64447	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
JJB17_RS23525	64505..64738	+	234	WP_000006003.1	DUF905 family protein	-
JJB17_RS23530	64799..66822	+	2024	Protein_77	ParB/RepB/Spo0J family partition protein	-
JJB17_RS23535	66891..67325	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
JJB17_RS23540	67322..68084	+	763	Protein_79	plasmid SOS inhibition protein A	-
-	68053..68277	+	225	NuclAT_0	-	-
-	68053..68277	+	225	NuclAT_0	-	-
-	68053..68277	+	225	NuclAT_0	-	-
-	68053..68277	+	225	NuclAT_0	-	-
-	68210..68275	-	66	-	-	Antitoxin
JJB17_RS24570	68263..68412	+	150	Protein_80	plasmid maintenance protein Mok	-
JJB17_RS23545	68354..68479	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
JJB17_RS23550	68798..69094	-	297	Protein_82	hypothetical protein	-
JJB17_RS23555	69394..69690	+	297	WP_001272251.1	hypothetical protein	-
JJB17_RS23560	69801..70622	+	822	WP_001234445.1	DUF932 domain-containing protein	-
JJB17_RS23565	70919..71566	-	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
JJB17_RS23570	71843..72226	+	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
JJB17_RS23575	72417..73103	+	687	WP_015059009.1	PAS domain-containing protein	-
JJB17_RS23580	73197..73424	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	floR / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / fosA3 / blaTEM-1B / blaCTX-M-55	-	1..108766	108766

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T185898 WP_001372321.1 NZ_CP067246:68354-68479 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T185898 NZ_CP089052:255052-255333 [Acinetobacter ursingii]
ATGGCTATTAAACTTCGCAAATGGGACAGTGCTGAGCATCTTAAAACAGAGGATGATATGCAGGCTTACTTACAAGCATG
TATTGAAGAATCTAATGGCGATGCCGCATTCATTGCAAAGGCTTTAGGTAACATTGCAAAAGCTAAAGGAATGGCTCAAT
TATCACGAGATACAGGCTTAGGCCGAGAAAGTCTTTATAAAGCACTTTCTGGTGATGTTAACCCTAGTTTTGATACTGTG
ATTAAAGTTGTAAAAGCACTGAATTTACGCTTAGCAATTTAA

Antitoxin

Nucleotide

• Length: 66 bp

>AT185898 NZ_CP067246:c68275-68210 [Escherichia coli]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin