Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3696383..3697182 Replicon chromosome
Accession NZ_CP067245
Organism Escherichia coli strain OW1E2

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag JJB17_RS17835 Protein ID WP_000347251.1
Coordinates 3696383..3696847 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag JJB17_RS17840 Protein ID WP_001307405.1
Coordinates 3696847..3697182 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJB17_RS17805 (3691384) 3691384..3691818 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
JJB17_RS17810 (3691836) 3691836..3692714 - 879 WP_001300474.1 PTS N-acetylgalactosamine transporter subunit IID -
JJB17_RS17815 (3692704) 3692704..3693483 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
JJB17_RS17820 (3693494) 3693494..3693967 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
JJB17_RS17825 (3693990) 3693990..3695270 - 1281 WP_000681903.1 tagatose-bisphosphate aldolase subunit KbaZ -
JJB17_RS17830 (3695519) 3695519..3696328 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
JJB17_RS17835 (3696383) 3696383..3696847 - 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
JJB17_RS17840 (3696847) 3696847..3697182 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
JJB17_RS17845 (3697331) 3697331..3698902 - 1572 WP_097463558.1 galactarate dehydratase -
JJB17_RS17850 (3699277) 3699277..3700611 + 1335 WP_130078161.1 galactarate/glucarate/glycerate transporter GarP -
JJB17_RS17855 (3700627) 3700627..3701397 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T185892 WP_000347251.1 NZ_CP067245:c3696847-3696383 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T185892 NZ_CP089050:2296-2586 [Acinetobacter ursingii]
ATGCCACAAGTAATTTTAACCAGAAACGCCCTTAATGATTTGAGCCGATTAAAGGTGTTTTTGCAGACAAAGAACCCAGA
AGCGGCTAAGGAAGCGGTTATAACTATCCGATCTCACCTCAAGGAATTAGCCACTACACCATTGGCTCATGTACCTGACG
ATGAGCGACCAGATTTTAGGATATTGATAATCCCTTTTGGTTCAAGTGGATACAAGGCTTGGTATCGTTACAAAACTGGT
GAGGATGTTGTGATAGTCTCAATCAAGCATCAACGAGAAGATGCGTATTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT185892 WP_001307405.1 NZ_CP067245:c3697182-3696847 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT185892 NZ_CP089050:2027-2308 [Acinetobacter ursingii]
ATGTCCACCATAGCACTGTCCATCAAGCTTGATTCAAGCTATAAAGACCGTTTAAGCCGTTTAGCCACCACCAAAAAGCG
TACAGCACACGCATTGGCAAAGGCTGCAATCACTAGTTTTATCGAGCAAGAGGAGCGCATTGAGGCAGAGCGGAGAGAAG
CTATAGCCTCGTATGAAGATTATCTGGAAACAGGGCTGCATGTAACTCTAGCAGAAGCGGATGAGTGGCTGGAGAGTTGG
GGAACTGACAATGAGAAAGATGCACCCCTATGCCACAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References