Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 2022686..2023227 Replicon chromosome
Accession NZ_CP067245
Organism Escherichia coli strain OW1E2

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag JJB17_RS09950 Protein ID WP_000615976.1
Coordinates 2022949..2023227 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag JJB17_RS09945 Protein ID WP_000729704.1
Coordinates 2022686..2022946 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJB17_RS09915 (2018047) 2018047..2018970 - 924 WP_001232547.1 putative lateral flagellar export/assembly protein LafU -
JJB17_RS09920 (2018973) 2018973..2019836 - 864 WP_001169518.1 flagellar motor stator protein MotA -
JJB17_RS09925 (2019849) 2019849..2020004 - 156 Protein_1969 sigma factor-like helix-turn-helix DNA-binding protein -
JJB17_RS09930 (2020073) 2020073..2021053 + 981 WP_000019445.1 IS5-like element ISKpn26 family transposase -
JJB17_RS09935 (2021201) 2021201..2021551 - 351 Protein_1971 REP-associated tyrosine transposase RayT -
JJB17_RS09940 (2021727) 2021727..2022500 - 774 WP_016245244.1 C40 family peptidase -
JJB17_RS09945 (2022686) 2022686..2022946 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
JJB17_RS09950 (2022949) 2022949..2023227 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
JJB17_RS09955 (2023383) 2023383..2024123 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
JJB17_RS09960 (2024094) 2024094..2024861 - 768 WP_001365365.1 class II glutamine amidotransferase -
JJB17_RS09965 (2025067) 2025067..2025645 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2020073..2021053 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T185889 WP_000615976.1 NZ_CP067245:2022949-2023227 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T185889 NZ_CP089045:c150708-150388 [Acinetobacter ursingii]
ATGTTATTTATTGAAACCAGCATCTTTACCAAGCAAATTAAAGACCTTGTATCTGATGAGGAATATCGTCAACTTCAGCA
AGATCTTTTGGTACAGCCTGATAGAGGTGACCTAATCAAGAATGGCGGTGGTATTCGCAAAGTACGTTGTGCTCAAGGTA
ACAAAGGGAAAAGTGGTGGGATACGGGTGATTTATTATTGGGTCACCGAGGATGATCAGATCTTTTTCTTAGTGGCTTAT
CCAAAATCAGTAAAAGATAATTTAACAGATAAGGAAACCGCCATTCTGCACCAACTCGTGAAGGAGCAATTTCATGGATA
A

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT185889 WP_000729704.1 NZ_CP067245:2022686-2022946 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT185889 NZ_CP089045:c150395-150123 [Acinetobacter ursingii]
ATGGATAACAACTTATTTGATGACTTGGTTGCTTCAATCAAAGAAGCTGGAGCTATCAAACGTAAAGAAGTTAAAGCAAG
CCGAGTTACAGAACTCGAATTGCCTGATATTAAAGAAGTACGTGAAAAAACTGGCTTGAGCCAAAATGAATTTGCTGCAC
GTCTACATATTAGCCCCCGTACCCTGCAAAATTGGGAACAGGGACGACGCTATCCAACTGGACCAGCAGCTACATTGATT
CGAATTTTAGATGCTCACCCAAGCCTTATTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References