Detailed information of TA system TA185873

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 113892..114125
• Replicon: plasmid pP19_598a
• Accession: NZ_CP067242
• Organism: Escherichia coli strain P19_598

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: JJB18_RS24580
• Protein ID: WP_001372321.1
• Coordinates: 113892..114017 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	114094..114125 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJB18_RS24545 (108938)	108938..109165	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
JJB18_RS24550 (109259)	109259..109945	-	687	WP_000332484.1	PAS domain-containing protein	-
JJB18_RS24555 (110136)	110136..110519	-	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
JJB18_RS24560 (110796)	110796..111443	+	648	WP_000614936.1	transglycosylase SLT domain-containing protein	-
JJB18_RS24565 (111740)	111740..112561	-	822	WP_001234469.1	DUF932 domain-containing protein	-
JJB18_RS24570 (112683)	112683..112970	-	288	WP_000107535.1	hypothetical protein	-
JJB18_RS24575 (112995)	112995..113201	-	207	WP_000547939.1	hypothetical protein	-
JJB18_RS25150 (113271)	113271..113444	+	174	Protein_140	hypothetical protein	-
JJB18_RS25155 (113442)	113442..113672	-	231	WP_001426396.1	hypothetical protein	-
JJB18_RS24580 (113892)	113892..114017	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
JJB18_RS25160 (113959)	113959..114108	-	150	Protein_143	plasmid maintenance protein Mok	-
- (114094)	114094..114125	-	32	NuclAT_1	-	Antitoxin
- (114094)	114094..114125	-	32	NuclAT_1	-	Antitoxin
- (114094)	114094..114125	-	32	NuclAT_1	-	Antitoxin
- (114094)	114094..114125	-	32	NuclAT_1	-	Antitoxin
- (115567)	115567..115764	-	198	NuclAT_0	-	-
- (115567)	115567..115764	-	198	NuclAT_0	-	-
- (115567)	115567..115764	-	198	NuclAT_0	-	-
- (115567)	115567..115764	-	198	NuclAT_0	-	-
JJB18_RS24590 (115576)	115576..115764	+	189	WP_001299721.1	hypothetical protein	-
JJB18_RS24595 (115733)	115733..116495	-	763	Protein_146	plasmid SOS inhibition protein A	-
JJB18_RS24600 (116492)	116492..116926	-	435	WP_000845895.1	conjugation system SOS inhibitor PsiB	-
JJB18_RS24605 (116981)	116981..118939	-	1959	WP_001145469.1	ParB/RepB/Spo0J family partition protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	floR / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / fosA3 / blaCTX-M-27 / dfrA14 / aadA2 / cmlA1 / ant(3'')-Ia / sul3 / aph(3')-Ia / mph(A) / sitABCD	-	1..144175	144175

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T185873 WP_001372321.1 NZ_CP067242:c114017-113892 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T185873 NZ_CP089036:3548449-3548736 [Escherichia coli O157:H7]
ATGTTACCCATTTTATGGCTACCGTCTGCTCGCGATGATTTGCGCCAGATCATAACTTACATCGCCAAGGAGAACCCACC
GGCAGCACGTAGACTAAAAATACGCATTGAAACATCGGTATTACCTCTATCTGAGCATCCGTACTTATATCCACCAAGCG
AACGGGTTTCTGGATTGAGAGAGATCGTGACCCACCCTAACTACATAATCCTGTACAGAGTAGCTGCTTCAAGCATTGAG
ATTGTAAGCGTGACACATTCTCGGCGACAATTTCCCTTCTCTATCTGA

Antitoxin

Nucleotide

• Length: 32 bp

>AT185873 NZ_CP067242:c114125-114094 [Escherichia coli]
CACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin