Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 3678353..3679032 Replicon chromosome
Accession NZ_CP067241
Organism Escherichia coli strain P19_598

Toxin (Protein)


Gene name higB Uniprot ID A0A0A1A1R1
Locus tag JJB18_RS17440 Protein ID WP_000057541.1
Coordinates 3678730..3679032 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag JJB18_RS17435 Protein ID WP_000806442.1
Coordinates 3678353..3678694 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJB18_RS17425 (3674596) 3674596..3675528 - 933 WP_000883039.1 glutaminase A -
JJB18_RS17430 (3675791) 3675791..3678295 + 2505 WP_061092784.1 copper-exporting P-type ATPase CopA -
JJB18_RS17435 (3678353) 3678353..3678694 - 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
JJB18_RS17440 (3678730) 3678730..3679032 - 303 WP_000057541.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JJB18_RS17445 (3679165) 3679165..3679959 + 795 WP_000365157.1 TraB/GumN family protein -
JJB18_RS17450 (3680163) 3680163..3680642 + 480 WP_000186626.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
JJB18_RS17455 (3680679) 3680679..3682331 - 1653 WP_000771723.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
JJB18_RS17460 (3682549) 3682549..3683769 + 1221 WP_001251634.1 fosmidomycin MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11795.37 Da        Isoelectric Point: 10.2638

>T185863 WP_000057541.1 NZ_CP067241:c3679032-3678730 [Escherichia coli]
MAQKNNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

>T185863 NZ_CP089036:940501-940908 [Escherichia coli O157:H7]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT185863 WP_000806442.1 NZ_CP067241:c3678694-3678353 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp

>AT185863 NZ_CP089036:940254-940520 [Escherichia coli O157:H7]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTATGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCCGATC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A1A1R1

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References