Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2456116..2456678 Replicon chromosome
Accession NZ_CP067241
Organism Escherichia coli strain P19_598

Toxin (Protein)


Gene name higB Uniprot ID L4J948
Locus tag JJB18_RS11455 Protein ID WP_000605676.1
Coordinates 2456400..2456678 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag JJB18_RS11450 Protein ID WP_000781370.1
Coordinates 2456116..2456400 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJB18_RS11435 (2451475) 2451475..2454522 + 3048 WP_012311994.1 formate dehydrogenase-N subunit alpha -
JJB18_RS11440 (2454535) 2454535..2455419 + 885 WP_001240582.1 formate dehydrogenase N subunit beta -
JJB18_RS11445 (2455412) 2455412..2456065 + 654 WP_000045650.1 formate dehydrogenase-N subunit gamma -
JJB18_RS11450 (2456116) 2456116..2456400 - 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
JJB18_RS11455 (2456400) 2456400..2456678 - 279 WP_000605676.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JJB18_RS11460 (2456864) 2456864..2457874 - 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
JJB18_RS11465 (2458008) 2458008..2459705 - 1698 WP_000433464.1 malate dehydrogenase -
JJB18_RS11470 (2459862) 2459862..2459999 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
JJB18_RS11475 (2460101) 2460101..2460316 - 216 WP_000495766.1 biofilm-dependent modulation protein -
JJB18_RS11480 (2460661) 2460661..2461092 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T185853 WP_000605676.1 NZ_CP067241:c2456678-2456400 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T185853 NZ_CP089034:c5098647-5098297 [Escherichia coli O157:H7]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GTGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTT
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT185853 WP_000781370.1 NZ_CP067241:c2456400-2456116 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT185853 NZ_CP089034:c5098892-5098641 [Escherichia coli O157:H7]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGTGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G6T1

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References