Detailed information of TA system TA185846

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 677845..678644
• Replicon: chromosome
• Accession: NZ_CP067241
• Organism: Escherichia coli strain P19_598

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: JJB18_RS03255
• Protein ID: WP_000347273.1
• Coordinates: 677845..678309 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: D6JF08
• Locus tag: JJB18_RS03260
• Protein ID: WP_001308975.1
• Coordinates: 678309..678644 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJB18_RS03225 (672846)	672846..673280	-	435	WP_000948834.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-
JJB18_RS03230 (673298)	673298..674176	-	879	WP_001298314.1	PTS N-acetylgalactosamine transporter subunit IID	-
JJB18_RS03235 (674166)	674166..674945	-	780	WP_208789578.1	PTS N-acetylgalactosamine transporter subunit IIC	-
JJB18_RS03240 (674956)	674956..675429	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
JJB18_RS03245 (675452)	675452..676732	-	1281	WP_000681961.1	tagatose-bisphosphate aldolase subunit KbaZ	-
JJB18_RS03250 (676981)	676981..677790	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
JJB18_RS03255 (677845)	677845..678309	-	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
JJB18_RS03260 (678309)	678309..678644	-	336	WP_001308975.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
JJB18_RS03265 (678793)	678793..680364	-	1572	WP_001273756.1	galactarate dehydratase	-
JJB18_RS03270 (680739)	680739..682073	+	1335	WP_000599651.1	galactarate/glucarate/glycerate transporter GarP	-
JJB18_RS03275 (682089)	682089..682859	+	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T185846 WP_000347273.1 NZ_CP067241:c678309-677845 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T185846 NZ_CP089034:c3380892-3380518 [Escherichia coli O157:H7]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12343.97 Da
• Isoelectric Point: 4.8616

>AT185846 WP_001308975.1 NZ_CP067241:c678644-678309 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT185846 NZ_CP089034:c3381313-3380939 [Escherichia coli O157:H7]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGAATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACCCGCAACCACCTCATAA

Structures

Toxin

• AlphaFold DB: V0SSH7

Antitoxin

• AlphaFold DB: D6JF08