Detailed information of TA system TA185838

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 97551..97977
• Replicon: plasmid pSBF22a
• Accession: NZ_CP067236
• Organism: Escherichia coli strain SBF22

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: JJB19_RS24425
• Protein ID: WP_001372321.1
• Coordinates: 97551..97676 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	97753..97977 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJB19_RS24400 (92919)	92919..93176	-	258	WP_000864318.1	conjugal transfer protein TrbE	-
JJB19_RS24405 (93203)	93203..95011	-	1809	WP_000821841.1	type-F conjugative transfer system mating-pair stabilization protein TraN	-
JJB19_RS24410 (95008)	95008..95646	-	639	WP_000777700.1	type-F conjugative transfer system pilin assembly protein TrbC	-
JJB19_RS24415 (95655)	95655..95993	-	339	Protein_111	TraU family protein	-
JJB19_RS24420 (96059)	96059..96763	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
JJB19_RS25715 (96931)	96931..97103	+	173	Protein_113	hypothetical protein	-
JJB19_RS25720 (97101)	97101..97331	-	231	WP_071586998.1	hypothetical protein	-
JJB19_RS24425 (97551)	97551..97676	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
JJB19_RS25725 (97618)	97618..97767	-	150	Protein_116	plasmid maintenance protein Mok	-
- (97753)	97753..97977	-	225	NuclAT_0	-	Antitoxin
- (97753)	97753..97977	-	225	NuclAT_0	-	Antitoxin
- (97753)	97753..97977	-	225	NuclAT_0	-	Antitoxin
- (97753)	97753..97977	-	225	NuclAT_0	-	Antitoxin
JJB19_RS24430 (97789)	97789..97977	+	189	WP_001299721.1	hypothetical protein	-
JJB19_RS24435 (97946)	97946..98708	-	763	Protein_118	plasmid SOS inhibition protein A	-
JJB19_RS24440 (98705)	98705..99139	-	435	WP_000845936.1	conjugation system SOS inhibitor PsiB	-
JJB19_RS24445 (99194)	99194..99391	-	198	Protein_120	hypothetical protein	-
JJB19_RS24450 (99419)	99419..99652	-	234	WP_000005987.1	DUF905 family protein	-
JJB19_RS24455 (99720)	99720..100259	-	540	WP_000290840.1	single-stranded DNA-binding protein	-
JJB19_RS24460 (100285)	100285..100491	-	207	WP_000275856.1	hypothetical protein	-
JJB19_RS25730 (100561)	100561..100641	+	81	Protein_124	hypothetical protein	-
JJB19_RS25735 (100824)	100824..100993	-	170	Protein_125	hypothetical protein	-
JJB19_RS24465 (101630)	101630..102601	-	972	WP_000817028.1	ParB/RepB/Spo0J family plasmid partition protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(A) / blaTEM-1B / tet(B) / catA1 / blaCTX-M-65 / fosA3 / sul2 / aph(3'')-Ib / aph(6)-Id / aph(3')-Ia / sitABCD	afaC-I / iutA / iucD / iucC / iucB / iucA	1..122982	122982

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T185838 WP_001372321.1 NZ_CP067236:c97676-97551 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T185838 NZ_CP089034:758193-758507 [Escherichia coli O157:H7]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTAGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTTTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Nucleotide

• Length: 225 bp

>AT185838 NZ_CP067236:c97977-97753 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin