Detailed information of TA system TA185825

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 2456402..2457201
• Replicon: chromosome
• Accession: NZ_CP067235
• Organism: Escherichia coli strain SBF22

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: JJB19_RS11725
• Protein ID: WP_000347273.1
• Coordinates: 2456737..2457201 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: V0TI39
• Locus tag: JJB19_RS11720
• Protein ID: WP_021547164.1
• Coordinates: 2456402..2456737 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJB19_RS11705 (2452187)	2452187..2452957	-	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-
JJB19_RS11710 (2452973)	2452973..2454307	-	1335	WP_000599651.1	galactarate/glucarate/glycerate transporter GarP	-
JJB19_RS11715 (2454682)	2454682..2456253	+	1572	WP_208776473.1	galactarate dehydratase	-
JJB19_RS11720 (2456402)	2456402..2456737	+	336	WP_021547164.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
JJB19_RS11725 (2456737)	2456737..2457201	+	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
JJB19_RS11730 (2457256)	2457256..2458065	-	810	WP_000072171.1	aga operon transcriptional regulator AgaR	-
JJB19_RS11735 (2458314)	2458314..2459594	+	1281	WP_000681958.1	tagatose-bisphosphate aldolase subunit KbaZ	-
JJB19_RS11740 (2459617)	2459617..2460090	+	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
JJB19_RS11745 (2460101)	2460101..2460880	+	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
JJB19_RS11750 (2460870)	2460870..2461748	+	879	WP_001295548.1	PTS N-acetylgalactosamine transporter subunit IID	-
JJB19_RS11755 (2461766)	2461766..2462200	+	435	WP_000948834.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2445919..2457201	11282

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T185825 WP_000347273.1 NZ_CP067235:2456737-2457201 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T185825 NZ_CP089032:4323124-4323342 [Escherichia coli O157:H7]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12309.86 Da
• Isoelectric Point: 4.5392

>AT185825 WP_021547164.1 NZ_CP067235:2456402-2456737 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDTQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT185825 NZ_CP089032:4322724-4323098 [Escherichia coli O157:H7]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACTCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: V0SSH7

Antitoxin

• AlphaFold DB: V0TI39