Detailed information of TA system TA185807

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 58977..59241
• Replicon: plasmid pSBF45a
• Accession: NZ_CP067233
• Organism: Escherichia coli strain SBF45

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Z417
• Locus tag: JJB20_RS22315
• Protein ID: WP_001387489.1
• Coordinates: 59089..59241 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	58977..59039 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJB20_RS22300 (54216)	54216..56507	-	2292	WP_021497839.1	F-type conjugative transfer protein TrbC	-
JJB20_RS22305 (56500)	56500..57570	-	1071	WP_000151583.1	IncI1-type conjugal transfer protein TrbB	-
JJB20_RS22310 (57589)	57589..58797	-	1209	WP_000121273.1	IncI1-type conjugal transfer protein TrbA	-
- (58977)	58977..59039	-	63	NuclAT_0	-	Antitoxin
- (58977)	58977..59039	-	63	NuclAT_0	-	Antitoxin
- (58977)	58977..59039	-	63	NuclAT_0	-	Antitoxin
- (58977)	58977..59039	-	63	NuclAT_0	-	Antitoxin
JJB20_RS22315 (59089)	59089..59241	+	153	WP_001387489.1	Hok/Gef family protein	Toxin
JJB20_RS22320 (59313)	59313..59564	-	252	WP_001291964.1	hypothetical protein	-
JJB20_RS23500 (60063)	60063..60158	+	96	WP_001303310.1	DinQ-like type I toxin DqlB	-
JJB20_RS22325 (60223)	60223..60399	-	177	WP_001054898.1	hypothetical protein	-
JJB20_RS22330 (60799)	60799..61008	+	210	WP_000062603.1	HEAT repeat domain-containing protein	-
JJB20_RS22335 (61080)	61080..61730	-	651	WP_001178506.1	plasmid IncI1-type surface exclusion protein ExcA	-
JJB20_RS22340 (61804)	61804..63972	-	2169	WP_054170920.1	DotA/TraY family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	fosA3	-	1..107006	107006

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5761.10 Da
• Isoelectric Point: 8.7948

>T185807 WP_001387489.1 NZ_CP067233:59089-59241 [Escherichia coli]
MPQRTFLMMLIVVCVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T185807 NZ_CP089031:c4837377-4837027 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GTGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTT
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Nucleotide

• Length: 63 bp

>AT185807 NZ_CP067233:c59039-58977 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: U9Z417

Antitoxin