Detailed information of TA system TA185788

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 1477320..1477922
• Replicon: chromosome
• Accession: NZ_CP067232
• Organism: Escherichia coli strain SBF45

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: JJB20_RS06930
• Protein ID: WP_000897305.1
• Coordinates: 1477611..1477922 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JJB20_RS06925
• Protein ID: WP_000356397.1
• Coordinates: 1477320..1477610 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJB20_RS06900 (1473246)	1473246..1474148	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
JJB20_RS06905 (1474145)	1474145..1474780	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
JJB20_RS06910 (1474777)	1474777..1475706	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
JJB20_RS06915 (1476036)	1476036..1476278	-	243	WP_001086388.1	protein YiiF	-
JJB20_RS06920 (1476497)	1476497..1476715	-	219	WP_001297075.1	CopG family transcriptional regulator	-
JJB20_RS06925 (1477320)	1477320..1477610	-	291	WP_000356397.1	NadS family protein	Antitoxin
JJB20_RS06930 (1477611)	1477611..1477922	-	312	WP_000897305.1	hypothetical protein	Toxin
JJB20_RS06935 (1478151)	1478151..1479059	+	909	WP_001162704.1	alpha/beta hydrolase	-
JJB20_RS06940 (1479123)	1479123..1480064	-	942	WP_001395735.1	fatty acid biosynthesis protein FabY	-
JJB20_RS06945 (1480109)	1480109..1480546	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
JJB20_RS06950 (1480543)	1480543..1481415	-	873	WP_000920762.1	virulence factor BrkB family protein	-
JJB20_RS06955 (1481409)	1481409..1482008	-	600	WP_001295269.1	glucose-1-phosphatase	-
JJB20_RS06960 (1482107)	1482107..1482892	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T185788 WP_000897305.1 NZ_CP067232:c1477922-1477611 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T185788 NZ_CP089031:c451239-450754 [Escherichia coli]
ATGGGAATAACGGCTCCTACTCCCCTAACGTCTGAACATAATCTGGCTGACTTTTGCTGTTCCGATCACGGGATGAATGA
ATGGCTAAAAAAGAAAGCGTTAAAAAATCACAGCTCCGGTCTGTCTCGTGTTTACGTTATCTGTATTGCCAATACCCGCC
AGGTAATCGGCTATTACTGCCTTTCTACAGGCAGTATTCAACGTAATTTGGCCCCTGGAGCCATGCGACGTAATGCTCCC
GAATCGTTGCCTGTTGTCGTATTAGGCAGACTGGCAATTGATCAAGCCTGGGCAGGTAAAGGGTTGGGCGTCGCATTGTT
GAAAGATGCAGTCTATCGCACAATGTCCATAGCTCAACAGGTAGGGGTTCGTGCGCTCATTGTTCATGCCTTAGACGATT
CAGTACGCAATTTCTATCTGAAGTATGCCTTTGTACCTTCACCATTCCAGTCACTTACCTTACTGTATCCCATTACTCTG
GAGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT185788 WP_000356397.1 NZ_CP067232:c1477610-1477320 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT185788 NZ_CP089031:c451508-451230 [Escherichia coli]
ATGAAACCGGAAAGCAAAGAAGCGCCAATCAATATCAGAGCGAAAGCCTCACAGCGTGACCTGATTGATATGGCAGCGAA
TCTGGTCGCAAAATCGCGCACTGATTTTATGCTGGATGCCGCATGCCGTGAAGCGCAAGACATCTTATTAGATCAACGAC
TATTCATCCTTGATGACGAGCAATACGATGCGTTTCTCGCTGCCCTGGACGCCCCCATCACTGCTGAGCGTCAGGCCAAA
ATTAACGCGTTGATGAATCGTAAATCGCCATGGGAATAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7