Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 648146..648687 Replicon chromosome
Accession NZ_CP067232
Organism Escherichia coli strain SBF45

Toxin (Protein)


Gene name yafQ Uniprot ID A7ZHY5
Locus tag JJB20_RS03155 Protein ID WP_012000916.1
Coordinates 648409..648687 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag JJB20_RS03150 Protein ID WP_000729704.1
Coordinates 648146..648406 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJB20_RS03125 (643182) 643182..644321 + 1140 WP_001402040.1 flagellar type III secretion system protein FlhB -
JJB20_RS03130 (644305) 644305..646398 + 2094 WP_001597626.1 flagellar biosynthesis protein FlhA -
JJB20_RS03135 (646503) 646503..646781 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
JJB20_RS03140 (646774) 646774..647130 + 357 WP_001030483.1 type II toxin-antitoxin system HicB family antitoxin -
JJB20_RS03145 (647187) 647187..647960 - 774 WP_000543899.1 C40 family peptidase -
JJB20_RS03150 (648146) 648146..648406 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
JJB20_RS03155 (648409) 648409..648687 + 279 WP_012000916.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
JJB20_RS03160 (648843) 648843..649583 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
JJB20_RS03165 (649554) 649554..650321 - 768 WP_000333380.1 class II glutamine amidotransferase -
JJB20_RS03170 (650527) 650527..651105 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T185785 WP_012000916.1 NZ_CP067232:648409-648687 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQSSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T185785 NZ_CP089031:277215-277322 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT185785 WP_000729704.1 NZ_CP067232:648146-648406 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT185785 NZ_CP089031:c277167-277101 [Escherichia coli]
GGTCTAGGTTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A7ZHY5

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References