Detailed information of TA system TA185763

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 4710962..4711575
• Replicon: chromosome
• Accession: NZ_CP067101
• Organism: Bradyrhizobium diazoefficiens strain 41_2

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: JJB98_RS22285
• Protein ID: WP_200455566.1
• Coordinates: 4711291..4711575 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JJB98_RS22280
• Protein ID: WP_200455565.1
• Coordinates: 4710962..4711279 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJB98_RS22255	4706246..4706737	+	492	WP_200455560.1	hypothetical protein	-
JJB98_RS22260	4706908..4707171	-	264	WP_200455561.1	EscU/YscU/HrcU family type III secretion system export apparatus switch protein	-
JJB98_RS22265	4707168..4708772	-	1605	WP_200455562.1	flagellar hook-length control protein FliK	-
JJB98_RS22270	4708828..4709622	-	795	WP_200455563.1	ATPase	-
JJB98_RS22275	4709751..4710965	-	1215	WP_200455564.1	RluA family pseudouridine synthase	-
JJB98_RS22280	4710962..4711279	-	318	WP_200455565.1	HigA family addiction module antidote protein	Antitoxin
JJB98_RS22285	4711291..4711575	-	285	WP_200455566.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JJB98_RS22290	4711632..4712966	-	1335	WP_200455567.1	replication-associated recombination protein A	-
JJB98_RS22295	4712963..4714369	-	1407	WP_200455568.1	DegQ family serine endoprotease	-
JJB98_RS22300	4714480..4714626	-	147	WP_200455569.1	hypothetical protein	-
JJB98_RS22305	4714681..4716282	+	1602	WP_200455570.1	OprO/OprP family phosphate-selective porin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-94)

Antitoxin

• Cro/C1-type helix-turn-helix domain (9-66)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10722.04 Da
• Isoelectric Point: 5.7105

>T185763 WP_200455566.1 NZ_CP067101:c4711575-4711291 [Bradyrhizobium diazoefficiens]
MIVSYRDKRTERFAAGQHVKEFSGFVRQAETRLDRLDAATSLADLAALPGNRLEALKGDRAGQFSIRINDQWRICFAWPE
ESQGPIDVEIVDYH

Nucleotide

• Length: 285 bp

>T185763 NZ_CP089021:c4764415-4763882 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCTGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11668.37 Da
• Isoelectric Point: 10.5812

>AT185763 WP_200455565.1 NZ_CP067101:c4711279-4710962 [Bradyrhizobium diazoefficiens]
MPRSPIHPGEQLAEELRELGITAAELARQIDVPVNRVTGIINGQRGITADTALRLGHWFGTTPQFWMNLQQQYELRLAEK
EVGAQVASLPRRANAQLTRKLGKTA

Nucleotide

• Length: 318 bp

>AT185763 NZ_CP089021:c4764692-4764426 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA