Detailed information of TA system TA185731

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4179690..4180292
• Replicon: chromosome
• Accession: NZ_CP067091
• Organism: Salmonella enterica subsp. enterica serovar Typhimurium strain ER3625

Toxin (Protein)

• Gene name: higB
• Uniprot ID: C0Q3J8
• Locus tag: JJB80_RS20285
• Protein ID: WP_001159630.1
• Coordinates: 4179690..4180001 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JJB80_RS20290
• Protein ID: WP_000362050.1
• Coordinates: 4180002..4180292 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJB80_RS20250 (4174803)	4174803..4175402	+	600	WP_000965695.1	glucose-1-phosphatase	-
JJB80_RS20255 (4175396)	4175396..4176268	+	873	WP_000921427.1	virulence factor BrkB family protein	-
JJB80_RS20260 (4176265)	4176265..4176702	+	438	WP_000560974.1	D-aminoacyl-tRNA deacylase	-
JJB80_RS20265 (4176747)	4176747..4177688	+	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
JJB80_RS20270 (4177703)	4177703..4178149	-	447	WP_001259011.1	type II toxin-antitoxin system HigA family antitoxin	-
JJB80_RS20275 (4178146)	4178146..4178457	-	312	WP_000558166.1	type II toxin-antitoxin system HigB family toxin	-
JJB80_RS20280 (4178543)	4178543..4179472	-	930	WP_001127703.1	alpha/beta hydrolase	-
JJB80_RS20285 (4179690)	4179690..4180001	+	312	WP_001159630.1	cytotoxic translational repressor of toxin-antitoxin stability system	Toxin
JJB80_RS20290 (4180002)	4180002..4180292	+	291	WP_000362050.1	DNA-binding transcriptional regulator	Antitoxin
JJB80_RS20295 (4180339)	4180339..4181268	-	930	WP_000027730.1	formate dehydrogenase accessory protein FdhE	-
JJB80_RS20300 (4181265)	4181265..4181900	-	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
JJB80_RS20305 (4181897)	4181897..4182799	-	903	WP_000331361.1	formate dehydrogenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12326.27 Da
• Isoelectric Point: 9.4460

>T185731 WP_001159630.1 NZ_CP067091:4179690-4180001 [Salmonella enterica subsp. enterica serovar Typhimurium]
MQFIETELFTEDVKKLLDDDEYHKLQVFMAQHPDCGDVIQETGGLRKMRWGARGKGKRSGVRIIYFHRSQRYEIRLLLIY
QKGIKDDLTPQEKAVLRMLNERW

Nucleotide

• Length: 312 bp

>T185731 NZ_CP089012:3860748-3860966 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10971.59 Da
• Isoelectric Point: 10.6525

>AT185731 WP_000362050.1 NZ_CP067091:4180002-4180292 [Salmonella enterica subsp. enterica serovar Typhimurium]
MDKVLFERLTQSMSQMNEIIEGTREPSRTFHIDAMKIKEIRQASGLSQSKFAELISVNVDTLRNWEQGRRSPTGPAKALL
RAIANDPRNVIQALRY

Nucleotide

• Length: 291 bp

>AT185731 NZ_CP089012:3860347-3860721 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: A0A0R9NS64