Detailed information of TA system TA185696

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-RelB
• Location: 2252561..2253095
• Replicon: chromosome
• Accession: NZ_CP067071
• Organism: Rhizobium sp. AB2/73

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: I8E17_RS11160
• Protein ID: WP_083258170.1
• Coordinates: 2252561..2252827 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: A0A1E4YBJ2
• Locus tag: I8E17_RS11165
• Protein ID: WP_069612752.1
• Coordinates: 2252835..2253095 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I8E17_RS11140 (I8E17_11125)	2248286..2248621	-	336	WP_069612748.1	hypothetical protein	-
I8E17_RS11145 (I8E17_11130)	2248835..2250073	+	1239	WP_069612749.1	urate hydroxylase PuuD	-
I8E17_RS11150 (I8E17_11135)	2250070..2251377	+	1308	WP_069612750.1	guanine deaminase	-
I8E17_RS11155 (I8E17_11140)	2251425..2252564	+	1140	WP_069612751.1	alpha-hydroxy acid oxidase	-
I8E17_RS11160 (I8E17_11145)	2252561..2252827	-	267	WP_083258170.1	Txe/YoeB family addiction module toxin	Toxin
I8E17_RS11165 (I8E17_11150)	2252835..2253095	-	261	WP_069612752.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
I8E17_RS11170 (I8E17_11155)	2253161..2253901	-	741	WP_069612753.1	metallophosphoesterase family protein	-
I8E17_RS11175 (I8E17_11160)	2253904..2254260	-	357	WP_069612754.1	hydroxyisourate hydrolase	-
I8E17_RS11180 (I8E17_11165)	2254257..2254757	-	501	WP_069612755.1	ureidoglycolate lyase	-
I8E17_RS11185 (I8E17_11170)	2254765..2255127	-	363	WP_069612756.1	DUF86 domain-containing protein	-
I8E17_RS11190 (I8E17_11175)	2255356..2255853	-	498	WP_069612757.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
I8E17_RS11195 (I8E17_11180)	2255855..2256778	-	924	WP_069612758.1	allantoinase PuuE	-
I8E17_RS11200 (I8E17_11185)	2256895..2257602	-	708	WP_069612759.1	DUF1045 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10807.46 Da
• Isoelectric Point: 9.9059

>T185696 WP_083258170.1 NZ_CP067071:c2252827-2252561 [Rhizobium sp. AB2/73]
MLLAWHAHAWEDYLYWRERDRKIAVRINELLRDALRHPFEGLGKPEALRNQLKGQWSRRITQEHRLIYKVEGEGDRQILI
VLQCRFHY

Nucleotide

• Length: 267 bp

>T185696 NZ_CP088997:2519832-2521154 [Klebsiella pneumoniae]
ATGGCGACGCTTACCACCTGGATGAACAATGTGCGCGTGGGAACCTTAACCCGCCAGGCTAACGGGGCGCACAGTTTTCG
CTATGACGAAGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCCCTCTCACTGCCGCTGCAGTATGGCAATATCACTG
CCGACGCGGTGTATCACTATTTTGATAACCTGCTGCCCGACAGCCCGCAGGTCCGCGACCGGATCGTCAGGCGCTATCAG
GCGCGCTCGAAGCAGCCGTTCGATCTGCTGGCCGAAGTCGGGCGGGATAGCGTAGGGGCGGTGACCCTCCTGCCGCCGGG
GGAAGAGGCGCACCTGGAAGGATTACGCTGGCAGACGCTGGACGAGGCGCAGCTCACCGCTTTGCTGACCGCTTACCAGT
CGGATATCCCCCTCGGGATGATTACCAGCCAGGACGATTTCCGTATCTCGGTGGCCGGCGCCCAGGAGAAAACGGCGCTG
CTGCGGATGGGCGAGCAGTGGTGTATTCCGCAGGGCGCCACGCCGACCACGCATATCATTAAGCTGCCCATCGGCGAGAT
CAAGCAGCCCAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAATGAGTATCTGTGCCTGGCGCTGGCCAGAGAACTGG
GCCTGGCGGTGCCGGAAGCGGAGATCATCACCACCCCGCGGATCCGCGCCCTGGCGGTAACCCGCTTCGACCGGCGCTGG
GCGCAGGAGGGACGGGTGCTGCTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCTTTTGGTCTTCCTTCAGCGATGAAATA
TGAGTCTGACGGCGGGCCGGGTATCGCCGCGATCATGACCTTTCTGCTGGGCTCGAGTGAAGCGCTGAAAGATCGCTATG
ACTTTATGAAGTTCATGGTCTTCCAGTGGCTGACGGGGGCGACGGACGGTCACGCAAAAAACTTCTCAATTTACCTGTTG
CCCGGCGGCAGCTACCGCTTAACACCGTTCTACGACATTATTTCGGCGTTTCCGGTGCTGGGCGGCACGGGACTGCATCT
GCGCGATCTGAAGCTGTCGATGGGGCTCAATGCCACAAAAGGGCGTAAAACAGAGATTAACGCCATCTATCCGCGTCATT
TTCTGGCGACGGCCAAAGCGGTGAACTTTCCCCTGGAGCAGATGCTGGCGATCCTCCAGGAGTTTGCCGGGCATGTGCCG
CAGGCAATCGAAAGCGCTCGCCGGACGCTGCCGGCGGACTTTTCCAGCCACGTCTGGCAGGCCATTACAGAGAACATGCT
GAAGCTGCACGCCCGCCTGCAGCAAGGGCTGCAGGCGCTATAG

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9557.92 Da
• Isoelectric Point: 4.5084

>AT185696 WP_069612752.1 NZ_CP067071:c2253095-2252835 [Rhizobium sp. AB2/73]
MRTVMFSAARNELASLLDEVVQDRVAVEIVRRDKPSAILIDKEEYEGIIETLHVLSTPANAARLMEAITDANEGKNLVSH
DLIDPK

Nucleotide

• Length: 261 bp

>AT185696 NZ_CP088997:2519566-2519832 [Klebsiella pneumoniae]
ATGAATTCCCCGCAGATATACAGTCCCGTGCAGCTGGCCAATTACCTGAAGCTGCTGCGCCAGCAGAACCACTGGACCCA
GGATGCGCTGGCCAGGCGGATCGGCATTAAGCAGGCCACGCTGTCCAATTTTGAAAATCACCCCGACAAAACCACCTTAA
CCACGCTGTTTAAGATCCTCCAGTCGCTGGGGGTGGTGATGACGCTGAACGCCAAAGCTGCTGAGACGGCGCCGGCTCCC
GATAACAACACGGACCTTGACTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A1E4YBJ2