Detailed information of TA system TA185673

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1060364..1061014
• Replicon: chromosome
• Accession: NZ_CP067066
• Organism: Raoultella sp. XY-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: JGY86_RS05050
• Protein ID: WP_200382415.1
• Coordinates: 1060673..1061014 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JGY86_RS05045
• Protein ID: WP_088402265.1
• Coordinates: 1060364..1060663 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JGY86_RS05035	1056777..1057511	+	735	WP_004866964.1	phosphoadenosine phosphosulfate reductase	-
JGY86_RS05040	1057566..1060271	-	2706	WP_099844278.1	CRISPR-associated helicase/endonuclease Cas3	-
JGY86_RS05045	1060364..1060663	-	300	WP_088402265.1	helix-turn-helix transcriptional regulator	Antitoxin
JGY86_RS05050	1060673..1061014	-	342	WP_200382415.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JGY86_RS05055	1061374..1062909	+	1536	WP_200382782.1	type I-E CRISPR-associated protein Cse1/CasA	-
JGY86_RS05060	1062926..1063528	+	603	WP_200382417.1	type I-E CRISPR-associated protein Cse2/CasB	-
JGY86_RS05065	1063551..1064609	+	1059	WP_099844213.1	type I-E CRISPR-associated protein Cas7/Cse4/CasC	-
JGY86_RS05070	1064619..1065344	+	726	WP_088402260.1	type I-E CRISPR-associated protein Cas5/CasD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-107)

Antitoxin

• HigA2-like, helix-turn-helix domain (9-88)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13036.70 Da
• Isoelectric Point: 4.8668

>T185673 WP_200382415.1 NZ_CP067066:c1061014-1060673 [Raoultella sp. XY-1]
MWDIETTDAFDEWFAAQTGTLQEDMLAAMVILSEYGPQLGRPFADTVNDSAFPNMKELRVQHQGNPIRAFFAFDPSRHGI
VLYAGDKTGLNEKKFYRDMLRLADNEYRKQLNK

Nucleotide

• Length: 342 bp

>T185673 NZ_CP088990:c154309-153866 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11048.85 Da
• Isoelectric Point: 8.6005

>AT185673 WP_088402265.1 NZ_CP067066:c1060663-1060364 [Raoultella sp. XY-1]
MATLKELMAKQSPESRERIAEKVEELRQTIALNMLREELNISQTELAAAMGVKQPTVAKMEQADNDPRLSTLKRYVTALG
GELSIDVKLPTGKRVAFHL

Nucleotide

• Length: 300 bp

>AT185673 NZ_CP088990:c154536-154306 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA