Detailed information of TA system TA185670

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 56914..57641
• Replicon: plasmid pZJ27003_p2
• Accession: NZ_CP067061
• Organism: Klebsiella pneumoniae strain ZJ27003

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: JE026_RS26320
• Protein ID: WP_011251285.1
• Coordinates: 57330..57641 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JE026_RS26315
• Protein ID: WP_011251286.1
• Coordinates: 56914..57333 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JE026_RS26285 (JE026_26250)	52082..52552	-	471	WP_048333570.1	hypothetical protein	-
JE026_RS26290 (JE026_26255)	52865..53500	-	636	WP_223171879.1	hypothetical protein	-
JE026_RS26295 (JE026_26260)	53919..54539	+	621	WP_223175004.1	conjugative transfer protein MobI(A/C)	-
JE026_RS26300 (JE026_26265)	54560..55348	+	789	WP_040217257.1	hypothetical protein	-
JE026_RS26305 (JE026_26270)	55362..55727	+	366	WP_048333448.1	hypothetical protein	-
JE026_RS26310 (JE026_26275)	55799..56767	-	969	WP_086642568.1	IS5 family transposase	-
JE026_RS26315 (JE026_26280)	56914..57333	-	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
JE026_RS26320 (JE026_26285)	57330..57641	-	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
JE026_RS26325 (JE026_26290)	57846..58283	-	438	Protein_58	DDE-type integrase/transposase/recombinase	-
JE026_RS26330 (JE026_26295)	58418..59115	+	698	WP_223175001.1	IS1-like element IS1A family transposase	-
JE026_RS26335 (JE026_26300)	59117..59566	+	450	WP_011251283.1	thioredoxin fold domain-containing protein	-
JE026_RS26340 (JE026_26305)	59583..59894	+	312	WP_011251282.1	hypothetical protein	-
JE026_RS26345 (JE026_26310)	59908..60249	+	342	WP_011251281.1	hypothetical protein	-
JE026_RS26350 (JE026_26315)	60309..61265	+	957	WP_011251280.1	DsbA family protein	-
JE026_RS26355 (JE026_26320)	61382..61957	+	576	WP_074424927.1	hypothetical protein	-
JE026_RS26360 (JE026_26325)	62065..62580	+	516	WP_041937820.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..141639	141639
	-	inside	IScluster/Tn	-	-	46793..59115	12322

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T185670 WP_011251285.1 NZ_CP067061:c57641-57330 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T185670 NZ_CP088989:42740-43045 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCTGTGAACTCTATCCGGTGG
TGCACATCGGGGATGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT185670 WP_011251286.1 NZ_CP067061:c57333-56914 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT185670 NZ_CP088989:42520-42738 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1