Detailed information of TA system TA185651

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 174085..174784
• Replicon: plasmid pPlas1
• Accession: NZ_CP067058
• Organism: Rahnella aceris strain ZF458

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: -
• Locus tag: JHW33_RS23265
• Protein ID: WP_200227412.1
• Coordinates: 174085..174381 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: A0A0H3FHC0
• Locus tag: JHW33_RS23270
• Protein ID: WP_013578313.1
• Coordinates: 174383..174784 (+)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JHW33_RS23235	170057..171013	-	957	WP_200227407.1	IS110 family transposase	-
JHW33_RS23240	171406..171912	+	507	WP_041689224.1	antirestriction protein ArdA	-
JHW33_RS23245	171954..172154	+	201	WP_013578318.1	hypothetical protein	-
JHW33_RS23250	172985..173227	+	243	WP_013578317.1	DUF905 family protein	-
JHW33_RS23255	173230..173535	+	306	WP_013578316.1	hypothetical protein	-
JHW33_RS23260	173532..173990	+	459	WP_200227410.1	hypothetical protein	-
JHW33_RS23265	174085..174381	+	297	WP_200227412.1	type II toxin-antitoxin system MqsR family toxin	Toxin
JHW33_RS23270	174383..174784	+	402	WP_013578313.1	type II toxin-antitoxin system MqsA family antitoxin	Antitoxin
JHW33_RS23275	175147..175338	+	192	WP_200227414.1	hypothetical protein	-
JHW33_RS23280	175449..175823	+	375	WP_200227417.1	hypothetical protein	-
JHW33_RS23285	175886..176227	+	342	WP_200227579.1	hypothetical protein	-
JHW33_RS23290	176316..176561	+	246	WP_200227420.1	hypothetical protein	-
JHW33_RS23295	176612..176986	+	375	WP_147423476.1	hypothetical protein	-
JHW33_RS23300	177212..177349	+	138	WP_200227422.1	hypothetical protein	-
JHW33_RS23305	178028..178378	+	351	WP_200227424.1	hypothetical protein	-
JHW33_RS23310	178429..178566	+	138	WP_200227427.1	hypothetical protein	-
JHW33_RS23315	178578..179201	+	624	WP_200227433.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..188256	188256

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 10770.41 Da
• Isoelectric Point: 7.8798

>T185651 WP_200227412.1 NZ_CP067058:174085-174381 [Rahnella aceris]
MEKKTPHTRLHIVQGFVAAGKVKATNTALNGADDLGLTFDDMCSAILGLTAADFFKSMTTYNDHTVWQDVYRPKLSCGDV
YLKLTVSNGVLIVSFKEK

Nucleotide

• Length: 297 bp

>T185651 NZ_CP088985:c4397378-4396845 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGTATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14922.38 Da
• Isoelectric Point: 8.4858

>AT185651 WP_013578313.1 NZ_CP067058:174383-174784 [Rahnella aceris]
MKCSVCGGAELEFGIKKVPYSFRGRKTVIEAEGDHCPACGEVVMDREQSMAYMAKVKSFKAKVIAETIEPEYIIQVRKKL
ELTQKEASEIFGGGVNAFSRYEKGKAQPHPSIIKLLRVLDHHPELLDEVRKQA

Nucleotide

• Length: 402 bp

>AT185651 NZ_CP088985:c4397655-4397389 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3FHC0