Detailed information of TA system TA185649

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-RelB
• Location: 62863..63371
• Replicon: plasmid pPlas1
• Accession: NZ_CP067058
• Organism: Rahnella aceris strain ZF458

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: H8P157
• Locus tag: JHW33_RS22715
• Protein ID: WP_013578404.1
• Coordinates: 62863..63129 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: H8P156
• Locus tag: JHW33_RS22720
• Protein ID: WP_013578405.1
• Coordinates: 63126..63371 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JHW33_RS22685	58392..59205	-	814	Protein_58	MFS transporter	-
JHW33_RS22690	59177..59622	+	446	Protein_59	DDE-type integrase/transposase/recombinase	-
JHW33_RS22695	59793..60425	+	633	WP_200227568.1	hypothetical protein	-
JHW33_RS22700	60454..61908	-	1455	Protein_61	ISNCY family transposase	-
JHW33_RS22705	62059..62430	-	372	WP_200227544.1	type II toxin-antitoxin system death-on-curing family toxin	-
JHW33_RS22710	62427..62648	-	222	WP_200227547.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
JHW33_RS22715	62863..63129	-	267	WP_013578404.1	Txe/YoeB family addiction module toxin	Toxin
JHW33_RS22720	63126..63371	-	246	WP_013578405.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
JHW33_RS22725	63683..64732	+	1050	WP_014683046.1	tyrosine-type recombinase/integrase	-
JHW33_RS22730	64824..65303	-	480	WP_014683045.1	GNAT family N-acetyltransferase	-
JHW33_RS22735	65300..65560	-	261	WP_200227549.1	DUF1778 domain-containing protein	-
JHW33_RS22740	65685..66023	+	339	Protein_69	MFS transporter	-
JHW33_RS22745	66409..67869	+	1461	WP_200227552.1	sensor domain-containing diguanylate cyclase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..188256	188256

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (1-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10490.04 Da
• Isoelectric Point: 10.0392

>T185649 WP_013578404.1 NZ_CP067058:c63129-62863 [Rahnella aceris]
MKTRFTKRGAADYQYWQGSNAKIFERIRILIHAIHADPLGGIGKPERLRHYKNPALYSRRIDREHRLVYSMVNDELTIIA
CRYHYDDV

Nucleotide

• Length: 267 bp

>T185649 NZ_CP088985:c3649680-3649363 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9027.06 Da
• Isoelectric Point: 4.1947

>AT185649 WP_013578405.1 NZ_CP067058:c63371-63126 [Rahnella aceris]
MHSLTFTDARKHFAETMQQVSDNAEPVRILRRDAPDVVLIDAAEYDAILETLYLFSNPANAAHINASLAELDRGDTVEIE
V

Nucleotide

• Length: 246 bp

>AT185649 NZ_CP088985:c3649370-3649083 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACAGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Structures

Toxin

• AlphaFold DB: H8P157

Antitoxin

• AlphaFold DB: H8P156