Detailed information of TA system TA185635

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1035764..1036449
• Replicon: chromosome
• Accession: NZ_CP067057
• Organism: Rahnella aceris strain ZF458

Toxin (Protein)

• Gene name: higB
• Uniprot ID: H8NS19
• Locus tag: JHW33_RS04750
• Protein ID: WP_013575753.1
• Coordinates: 1036135..1036449 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JHW33_RS04745
• Protein ID: WP_015690029.1
• Coordinates: 1035764..1036081 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JHW33_RS04725	1031507..1032385	+	879	WP_013575758.1	ABC transporter permease	-
JHW33_RS04730	1032375..1033259	+	885	WP_112152546.1	ABC transporter ATP-binding protein	-
JHW33_RS04735	1033252..1033929	+	678	WP_200225197.1	ATP-binding cassette domain-containing protein	-
JHW33_RS04740	1034072..1035649	+	1578	WP_200225198.1	ABC transporter substrate-binding protein	-
JHW33_RS04745	1035764..1036081	-	318	WP_015690029.1	HigA family addiction module antidote protein	Antitoxin
JHW33_RS04750	1036135..1036449	-	315	WP_013575753.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JHW33_RS04755	1036863..1038566	+	1704	WP_200225199.1	chloride channel protein	-
JHW33_RS04760	1038588..1039607	-	1020	WP_013575750.1	transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (6-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-70)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12595.39 Da
• Isoelectric Point: 9.2702

>T185635 WP_013575753.1 NZ_CP067057:c1036449-1036135 [Rahnella aceris]
MNDMLRISSFRDQWLEDFFHFATPHRKIPWTLATALERKLDMINAAMDYRDLLWPPGNRYEDLAPPLKGFSSIRVNEQYR
LIFYWREGKAHGLYLDPHGYRSYR

Nucleotide

• Length: 315 bp

>T185635 NZ_CP088982:4172671-4172889 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 12115.02 Da
• Isoelectric Point: 8.6390

>AT185635 WP_015690029.1 NZ_CP067057:c1036081-1035764 [Rahnella aceris]
MAVTTRKPATVGDILLYEFLEPAGLRINDLADMLNVHRNTISALVNNNRKLTVDIAFRLAKTFETSVEFWLNLQTNVDIW
EVRNNPRLMDDINKVAHLKDFMKKS

Nucleotide

• Length: 318 bp

>AT185635 NZ_CP088982:4172270-4172644 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: H8NS19