Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3956055..3956652 Replicon chromosome
Accession NZ_CP067045
Organism Klebsiella pneumoniae strain B6753

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag IL988_RS19545 Protein ID WP_004142563.1
Coordinates 3956335..3956652 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag IL988_RS19540 Protein ID WP_004142561.1
Coordinates 3956055..3956342 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IL988_RS19510 (3952136) 3952136..3952384 + 249 WP_002893037.1 DUF1158 domain-containing protein -
IL988_RS19515 (3952402) 3952402..3952743 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
IL988_RS19520 (3952774) 3952774..3953889 - 1116 WP_020316605.1 MBL fold metallo-hydrolase -
IL988_RS19525 (3954068) 3954068..3954649 + 582 WP_134901480.1 TetR/AcrR family transcriptional regulator -
IL988_RS19530 (3954649) 3954649..3955017 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
IL988_RS19535 (3955137) 3955137..3955790 + 654 WP_134901481.1 oxygen-insensitive NAD(P)H nitroreductase -
IL988_RS19540 (3956055) 3956055..3956342 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
IL988_RS19545 (3956335) 3956335..3956652 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
IL988_RS19550 (3956837) 3956837..3957880 - 1044 WP_032419264.1 DUF2157 domain-containing protein -
IL988_RS19555 (3958547) 3958547..3959413 - 867 WP_032419265.1 helix-turn-helix transcriptional regulator -
IL988_RS19560 (3959522) 3959522..3960949 + 1428 WP_009308097.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T185626 WP_004142563.1 NZ_CP067045:c3956652-3956335 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T185626 NZ_CP088980:721986-722267 [Rhodanobacter denitrificans]
GTGATAAGGAGCTTCGCTGACAAGGAAACCGCGCGACTGTCCGCGACCGGAACAAGCCGGCGGTTTCCGCCGGACATCGT
GCGGCGGGCGCTGAGTCGTCTGAAATATTTGCATCTCGCCACCTGCCCCGAAGATCTGCGCGAGCCGTTTTCCAATCGCC
TGGAAATGTTGAGCGGCAATCGCGCAGGTCAATGGAGTATCCGCATCAACGATCAGTGGCGGCTGTGCTTCCGTTTCGAG
GGCGGCGATGCCTTCGACGTCGAGATCGTCGACTACCATTGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT185626 WP_004142561.1 NZ_CP067045:c3956342-3956055 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT185626 NZ_CP088980:722280-722585 [Rhodanobacter denitrificans]
ATGTCCATCCCCGCCACCACGAACGGCCTGCCGCCCATTCATCCGGGGGAACTCCTGGAAGAAATCCTGTCCGACCACGG
CATGAGCCAGGCGGCATTTGCCCGGGCGATCGGCGTTTCGCCGATGCGTATTTCGCATGTCATCCGCGAGGAGCGCCCCG
TCACGGCCGAACTTGCCCTGCGCTTTGGCCGCGCCTTCGGCCAGAGCCCGCAGTTCTGGATGAACATGCAGACCGACTAC
GACCTCAAGATCGCCGAGCGTGAACTGAAGGCGAGTTTGCGGAAAGTTCTGCCATTGGCGGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References