Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 6025567..6026160 Replicon chromosome
Accession NZ_CP067024
Organism Pseudomonas syringae pv. maculicola strain MAFF 302723

Toxin (Protein)


Gene name graT Uniprot ID Q88B20
Locus tag JHZ65_RS27080 Protein ID WP_011103035.1
Coordinates 6025882..6026160 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name graA Uniprot ID Q88B19
Locus tag JHZ65_RS27075 Protein ID WP_005739893.1
Coordinates 6025567..6025872 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JHZ65_RS27045 6021256..6021948 + 693 WP_007246534.1 HAD family hydrolase -
JHZ65_RS27050 6022023..6022355 - 333 WP_007246533.1 hypothetical protein -
JHZ65_RS27055 6022454..6022879 - 426 WP_005768513.1 secondary thiamine-phosphate synthase enzyme YjbQ -
JHZ65_RS27060 6023121..6024458 - 1338 WP_005739890.1 ammonium transporter -
JHZ65_RS27065 6024494..6024832 - 339 WP_002555808.1 P-II family nitrogen regulator -
JHZ65_RS27070 6025252..6025533 + 282 WP_005768515.1 accessory factor UbiK family protein -
JHZ65_RS27075 6025567..6025872 - 306 WP_005739893.1 HigA family addiction module antidote protein Antitoxin
JHZ65_RS27080 6025882..6026160 - 279 WP_011103035.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JHZ65_RS27085 6026412..6027347 - 936 WP_011103034.1 LysR family transcriptional regulator -
JHZ65_RS27090 6027502..6028785 + 1284 WP_122389072.1 lysine N(6)-hydroxylase/L-ornithine N(5)-oxygenase family protein -
JHZ65_RS27095 6028785..6029660 + 876 WP_005768527.1 DMT family transporter -
JHZ65_RS27100 6029662..6030900 + 1239 WP_005768528.1 diaminopimelate decarboxylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(5-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(6-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10453.11 Da        Isoelectric Point: 9.1490

>T185594 WP_011103035.1 NZ_CP067024:c6026160-6025882 [Pseudomonas syringae pv. maculicola]
MIVSFKCVHTRYLFLQGKTRLWPSIKSVAERKLAMLDAATSILDLRSPPGNRLEALDGSRSGQYSVRINAPFRICFVWSI
NGPEDVEIVDYH

Download         Length: 279 bp

>T185594 NZ_CP088925:2226457-2226738 [Rhodanobacter denitrificans]
ATGACCATCCAGTCGTTCCGCTGCAAGGATACGCAGGCGCTGTTCGAGCGACGCCGGGTGCGGCGCTTTCTGGTGATCGA
GGCGGCGGCGCGGCGCAAGCTGGAGCTGTTGAATGCGGCGACCCAGCTGGACTATCTGCGGGTGCCGCCCGGCAATCGAC
TGGAAGCCTTGGCCGGCGATCGCAAGGGTCAGCACAGCATTCGTGTGAATGACCAGTTCCGGCTGTGCTTCGTGTGGACC
GCCGACGGCCCGGGCCTGGTCGAGATCGTCGATTATCACTGA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11158.01 Da        Isoelectric Point: 7.7509

>AT185594 WP_005739893.1 NZ_CP067024:c6025872-6025567 [Pseudomonas syringae pv. maculicola]
MNKNGMRPVHPGEVLKEEYLEPMGLTAAALARALKVSTPTVNDIVLQRRGVSADVALRLAVCLETSPEFWLNLQLAYDLR
KAETEKGAQIREQVKCLAHCA

Download         Length: 306 bp

>AT185594 NZ_CP088925:2226748-2227047 [Rhodanobacter denitrificans]
ATGCCCCGCAAACTGCCACCCATCCACCCCGGCGAGATCCTGCGCGAGGAATACATGCTGCCGCTGGAGCTGTCGTCCAA
CGCGCTGGCCCGCGCCCTCGAAGTCACGCCTGCACGTATCAACGAGATCGTGCGCGAGCGGCGCGGCATCAGCGCCGATA
CCGCGCTGCGGCTGGCGCGCTATTTCGGCAATGACGCGCAGAGCTGGCTCAACCTGCAGCAGCGCTACGACATCGAGTGC
GCGCAGGACAGCATCGGCGGCGCGCTGAGGAAAATCACGCCCTATCAGGCAGCCGCCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q88B20

Antitoxin

Source ID Structure
AlphaFold DB A0A0K8LYY6

References