Detailed information of TA system TA185583

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 215807..216392
• Replicon: plasmid unnamed2
• Accession: NZ_CP067021
• Organism: Acinetobacter sp. CS-2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A3F3L0I7
• Locus tag: JFY49_RS17120
• Protein ID: WP_039253786.1
• Coordinates: 216072..216392 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JFY49_RS17115
• Protein ID: WP_000369781.1
• Coordinates: 215807..216079 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JFY49_RS17095	211204..212079	+	876	WP_114190151.1	LysR family transcriptional regulator	-
JFY49_RS17100	212012..212716	+	705	WP_004897650.1	IS6-like element IS1006 family transposase	-
JFY49_RS17105	212898..213782	-	885	WP_000155092.1	Mph(E) family macrolide 2'-phosphotransferase	-
JFY49_RS17110	213838..215313	-	1476	WP_000052512.1	ABC-F type ribosomal protection protein Msr(E)	-
JFY49_RS17115	215807..216079	-	273	WP_000369781.1	helix-turn-helix domain-containing protein	Antitoxin
JFY49_RS17120	216072..216392	-	321	WP_039253786.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JFY49_RS17125	216521..217855	-	1335	WP_108091255.1	ISNCY family transposase	-
JFY49_RS17130	218017..218436	+	420	WP_001180106.1	SMI1/KNR4 family protein	-
JFY49_RS17135	218516..218797	-	282	WP_000985609.1	putative addiction module antidote protein	-
JFY49_RS17140	218798..219100	-	303	WP_000286964.1	type II toxin-antitoxin system RelE/ParE family toxin	-
JFY49_RS17145	219249..219779	+	531	WP_000172759.1	hypothetical protein	-
JFY49_RS17150	219868..220182	-	315	WP_004728120.1	BrnA antitoxin family protein	-
JFY49_RS17155	220169..220456	-	288	WP_000438825.1	BrnT family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(3')-VI / blaNDM-1 / floR / mph(E) / msr(E) / sul2 / blaOXA-58 / aac(3)-IId	-	1..283930	283930
	-	inside	IScluster/Tn	floR / mph(E) / msr(E) / sul2	-	206845..227467	20622

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12207.00 Da
• Isoelectric Point: 7.9849

>T185583 WP_039253786.1 NZ_CP067021:c216392-216072 [Acinetobacter sp. CS-2]
MLFIETSIFTKQIKDLVSDEEYRQLQQDLLVQPDRGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETAILRQLVKEQFHG

Nucleotide

• Length: 321 bp

>T185583 NZ_CP088922:c614020-613844 [Rhodanobacter denitrificans]
ATGAAGTACAGCGAGTTCCAGCGGTGGCTCAGGCAGCAGGGAGTGAGCTTCGAGCCCGGCAAGGGCAGCCACCAGCTGGC
GCGCTACAGAGGCCGGACTTCCGTCTTTCCCTTCCACGGATCGAAAGAGATGCCCGAGCCGCTGCGCAAGAAGATCCTGA
AGGACCTGGGTCTTTGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10149.61 Da
• Isoelectric Point: 8.6582

>AT185583 WP_000369781.1 NZ_CP067021:c216079-215807 [Acinetobacter sp. CS-2]
MDNNLFDDLVASIKEAGAIKRKEVKASRVTELELPDIKEVREKTGLSQNEFAARLHISPRTLQNWEQGRRYPTGPAATLI
RILDAHPSLI

Nucleotide

• Length: 273 bp

>AT185583 NZ_CP088922:c613811-613392 [Rhodanobacter denitrificans]
ATGAAATACCCCGTCAAGCTGACCCGTGCCGATAGCGTGGTGATGGTGACCTGCCCGGACCTGCCGGAGTTTTCCAGCGT
CGGCGACACCGAGGGCGAGGCTCTGCGCGAAGCCGTCGACGGTATCGAAACCACGCTGCAGGCGTACATGCAGGACCGGC
GTGACATTCCGCCGCCGGGCGCGCGCAAGCGCGGCCAGCGGCTGGTGGAGCTGCCGCCGCTGGCGGTGGCCAAGCTCGGT
CTGTACCGGGCCATGCGCGAACAGGGGTTGCGCAAGGCTGACCTGGCCCGCCGGCTCGACGTGCATCTGCCCCAGGTCGA
CCGCCTGCTCGACCTGCGCCACAGCTCGAAGCTGGAACAGGTGGAGGCGGCGCTCAACGCGTTGGGCTACCGGATCGAAC
TGGCGGTGCTGGCGGCGTAG

Structures

Toxin

• AlphaFold DB: A0A3F3L0I7