Detailed information of TA system TA185441

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 53104..53357
• Replicon: plasmid pXHKPN391-1
• Accession: NZ_CP066916
• Organism: Klebsiella pneumoniae strain XHKPN391

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: JG797_RS28355
• Protein ID: WP_001312851.1
• Coordinates: 53208..53357 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	53104..53163 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JG797_RS28315 (48764)	48764..48829	-	66	Protein_67	helix-turn-helix domain-containing protein	-
JG797_RS28320 (48882)	48882..49586	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
JG797_RS28325 (49611)	49611..49811	+	201	WP_072354025.1	hypothetical protein	-
JG797_RS28330 (49831)	49831..50577	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
JG797_RS28335 (50632)	50632..51192	+	561	WP_000139328.1	fertility inhibition protein FinO	-
JG797_RS28340 (51324)	51324..51524	+	201	WP_015059022.1	hypothetical protein	-
JG797_RS28345 (51910)	51910..52509	+	600	WP_032083981.1	PIN domain-containing protein	-
JG797_RS28350 (52571)	52571..52903	+	333	WP_152916585.1	hypothetical protein	-
- (53104)	53104..53163	-	60	NuclAT_0	-	Antitoxin
- (53104)	53104..53163	-	60	NuclAT_0	-	Antitoxin
- (53104)	53104..53163	-	60	NuclAT_0	-	Antitoxin
- (53104)	53104..53163	-	60	NuclAT_0	-	Antitoxin
JG797_RS28355 (53208)	53208..53357	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
JG797_RS28360 (53641)	53641..53889	+	249	WP_000083837.1	replication regulatory protein RepA	-
JG797_RS28365 (54004)	54004..54182	+	179	Protein_77	protein CopA/IncA	-
JG797_RS28370 (54201)	54201..55058	+	858	WP_142295464.1	incFII family plasmid replication initiator RepA	-
JG797_RS28375 (55997)	55997..56650	+	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
JG797_RS28380 (56743)	56743..57000	+	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
JG797_RS28385 (56933)	56933..57334	+	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
JG797_RS28390 (57583)	57583..57998	+	416	Protein_82	IS1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaCTX-M-65 / fosA3 / blaKPC-2	-	1..87509	87509

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T185441 WP_001312851.1 NZ_CP066916:53208-53357 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T185441 NZ_CP088221:c1091809-1091591 [Enterobacter hormaechei]
ATGTCTGATAAACCATTAACAAAAGTCGATTATTTGATGCGCCTGCGACGCTGCCAGTCAATTGACACCCTCGAACGTGT
TATTGAAAAAAATAAATACGAGCTGTCTGATAATGAACTGGCGGTATTTTATTCAGCTGCCGACCATCGTCTGGCTGAAC
TAACCATGAATAAACTGTATGACAAGATCCCCTCTTCGGTATGGAAATTTGTTCGTTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT185441 NZ_CP066916:c53163-53104 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin