Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 355289..355935 Replicon chromosome
Accession NZ_CP066915
Organism Klebsiella pneumoniae strain XHKPN391

Toxin (Protein)


Gene name higB Uniprot ID W8UNE1
Locus tag JG797_RS01640 Protein ID WP_002920560.1
Coordinates 355289..355636 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID W8UB68
Locus tag JG797_RS01645 Protein ID WP_002920557.1
Coordinates 355636..355935 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JG797_RS01630 (351215) 351215..352648 + 1434 WP_002920564.1 glycogen synthase GlgA -
JG797_RS01635 (352666) 352666..355113 + 2448 WP_002920561.1 glycogen phosphorylase -
JG797_RS01640 (355289) 355289..355636 + 348 WP_002920560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JG797_RS01645 (355636) 355636..355935 + 300 WP_002920557.1 helix-turn-helix transcriptional regulator Antitoxin
JG797_RS01650 (355998) 355998..357506 - 1509 WP_002920554.1 glycerol-3-phosphate dehydrogenase -
JG797_RS01655 (357711) 357711..358040 + 330 WP_002920552.1 thiosulfate sulfurtransferase GlpE -
JG797_RS01660 (358091) 358091..358921 + 831 WP_004151408.1 rhomboid family intramembrane serine protease GlpG -
JG797_RS01665 (358971) 358971..359729 + 759 WP_002920548.1 DeoR/GlpR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element blaSHV-12 / blaOXA-1 / aac(6')-Ib-cr rmpA / iutA / iucD / iucC / iucB / iucA 238617..482556 243939


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

HigA2-like, helix-turn-helix domain

(9-87)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13502.56 Da        Isoelectric Point: 6.2300

>T185424 WP_002920560.1 NZ_CP066915:355289-355636 [Klebsiella pneumoniae]
MWDVETTDTFDAWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRACFAFDPLRKAI
VLCVGNKDGMNEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

>T185424 NZ_CP088200:2772637-2772777 [Enterococcus faecalis]
ATTTCTCTGAAAAATACGAATAAAAATATCGTTTACTGTACAACATATGAAACAATTCAGACGATCCTAGGGTTTGGTAT
GTTTACCATTGCTTTGATTGCACTGATTGTGAAATTGCTTAAAAATGACAAGAAAAAATAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10800.58 Da        Isoelectric Point: 10.1464

>AT185424 WP_002920557.1 NZ_CP066915:355636-355935 [Klebsiella pneumoniae]
MATLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT185424 NZ_CP088200:c2772908-2772710 [Enterococcus faecalis]
TTTTTAGGTAATTGTGCTATAATGGTAATGAAAAGAGAGCTATGCTGTAACATACCTCTCTGATGTAGAGCCGTTTAAGA
CGGTGACCTTTTTGATTATTTAAAAATAACCGTACTGGTCAAAGTAGACGGTTATTTTTTCTTGTCATTTTTAAGCAATT
TCACAATCAGTGCAATCAAAGCAATGGTAAACATACCAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E1CBY5

Antitoxin

Source ID Structure
AlphaFold DB A0A0E1CBF8

References