Detailed information of TA system TA185414

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 59833..60259
• Replicon: plasmid pXHKPN083-2
• Accession: NZ_CP066912
• Organism: Klebsiella pneumoniae strain XHKPN083

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: JG792_RS28310
• Protein ID: WP_001372321.1
• Coordinates: 59833..59958 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	60035..60259 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JG792_RS28275 (54888)	54888..55115	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
JG792_RS28280 (55209)	55209..55895	-	687	WP_015059009.1	PAS domain-containing protein	-
JG792_RS28285 (56086)	56086..56469	-	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
JG792_RS28290 (56746)	56746..57393	+	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
JG792_RS28295 (57690)	57690..58511	-	822	WP_001234445.1	DUF932 domain-containing protein	-
JG792_RS28300 (58622)	58622..58918	-	297	WP_001272251.1	hypothetical protein	-
JG792_RS28305 (59218)	59218..59514	+	297	Protein_71	hypothetical protein	-
JG792_RS28310 (59833)	59833..59958	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
JG792_RS28315 (59900)	59900..60049	-	150	Protein_73	plasmid maintenance protein Mok	-
- (60035)	60035..60259	-	225	NuclAT_0	-	Antitoxin
- (60035)	60035..60259	-	225	NuclAT_0	-	Antitoxin
- (60035)	60035..60259	-	225	NuclAT_0	-	Antitoxin
- (60035)	60035..60259	-	225	NuclAT_0	-	Antitoxin
JG792_RS28320 (60271)	60271..60990	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
JG792_RS28325 (60987)	60987..61421	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
JG792_RS28330 (61490)	61490..63513	-	2024	Protein_76	ParB/RepB/Spo0J family partition protein	-
JG792_RS28335 (63574)	63574..63807	-	234	WP_000006003.1	DUF905 family protein	-
JG792_RS28340 (63865)	63865..64392	-	528	WP_000290834.1	single-stranded DNA-binding protein	-
JG792_RS28345 (64694)	64694..65155	+	462	Protein_79	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	catA2 / blaKPC-2 / rmtB / blaTEM-1B / blaCTX-M-65	-	1..122553	122553

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T185414 WP_001372321.1 NZ_CP066912:c59958-59833 [Klebsiella pneumoniae]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T185414 NZ_CP088198:2622078-2622221 [Enterococcus faecalis]
ATGAATGTAAGTGCTAAAATCTATGAAAGGAGAGGCCTTTTGTCAGCATATGAAACAATTCAGACGATCCTAGGGTTTGG
TATGTTTACCATTGCTTTGATTGCGCTGATTGTGAAATTGCTTAAAAATGACAAGAAAAAATAA

Antitoxin

Nucleotide

• Length: 225 bp

>AT185414 NZ_CP066912:c60259-60035 [Klebsiella pneumoniae]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin