Detailed information of TA system TA185387

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 45760..46013
• Replicon: plasmid pXHKP502-2
• Accession: NZ_CP066906
• Organism: Klebsiella pneumoniae strain XHKP502

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: JG791_RS28625
• Protein ID: WP_001312851.1
• Coordinates: 45760..45909 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	45954..46013 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JG791_RS28590 (41119)	41119..41534	-	416	Protein_53	IS1 family transposase	-
JG791_RS28595 (41783)	41783..42184	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
JG791_RS28600 (42117)	42117..42374	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
JG791_RS28605 (42467)	42467..43120	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
JG791_RS28610 (44059)	44059..44916	-	858	WP_032495102.1	incFII family plasmid replication initiator RepA	-
JG791_RS28615 (44935)	44935..45113	-	179	Protein_58	protein CopA/IncA	-
JG791_RS28620 (45228)	45228..45476	-	249	WP_000083837.1	replication regulatory protein RepA	-
JG791_RS28625 (45760)	45760..45909	-	150	WP_001312851.1	Hok/Gef family protein	Toxin
- (45954)	45954..46013	+	60	NuclAT_0	-	Antitoxin
- (45954)	45954..46013	+	60	NuclAT_0	-	Antitoxin
- (45954)	45954..46013	+	60	NuclAT_0	-	Antitoxin
- (45954)	45954..46013	+	60	NuclAT_0	-	Antitoxin
JG791_RS28630 (46214)	46214..46546	-	333	WP_152916585.1	hypothetical protein	-
JG791_RS28635 (46608)	46608..47207	-	600	WP_032083981.1	PIN domain-containing protein	-
JG791_RS28640 (47593)	47593..47793	-	201	WP_015059022.1	hypothetical protein	-
JG791_RS28645 (47925)	47925..48485	-	561	WP_000139328.1	fertility inhibition protein FinO	-
JG791_RS28650 (48540)	48540..49286	-	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
JG791_RS28655 (49306)	49306..49506	-	201	WP_072354025.1	hypothetical protein	-
JG791_RS28660 (49531)	49531..50235	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
JG791_RS28665 (50287)	50287..50631	+	345	Protein_68	IS6-like element IS26 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaKPC-2 / blaSHV-12 / blaCTX-M-65	-	1..85781	85781

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T185387 WP_001312851.1 NZ_CP066906:c45909-45760 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T185387 NZ_CP088157:c1580528-1580283 [Staphylococcus aureus]
ATGAACATTCAAGAAGCAACTAAGATAGCTACAAAAAATCTTGTCTCTATGACACGGAAAGATTGGAAAGAAAGTCATCG
AACTAAGATATTACCAACAAATGATAGTTTTTTACAATGCATCATTTCAAATAGCGATGGGACAAACCTTATCAGATATT
GGCAACCTTCAGCCGATGACCTCATGGCAAATGATTGGGAAGTTATAAACCCAACTAGAGACCAGGAATTATTGAAGCAA
TTTTAG

Antitoxin

Nucleotide

• Length: 60 bp

>AT185387 NZ_CP066906:45954-46013 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin