Detailed information of TA system TA185383

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 11449..12176
• Replicon: plasmid pXHKP502-1
• Accession: NZ_CP066905
• Organism: Klebsiella pneumoniae strain XHKP502

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: JG791_RS27190
• Protein ID: WP_011251285.1
• Coordinates: 11865..12176 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JG791_RS27185
• Protein ID: WP_011251286.1
• Coordinates: 11449..11868 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JG791_RS27155	6617..7087	-	471	WP_048333570.1	hypothetical protein	-
JG791_RS27160	7400..8035	-	636	WP_223171879.1	hypothetical protein	-
JG791_RS27165	8454..9074	+	621	WP_223175004.1	conjugative transfer protein MobI(A/C)	-
JG791_RS27170	9095..9883	+	789	WP_040217257.1	hypothetical protein	-
JG791_RS27175	9897..10262	+	366	WP_048333448.1	hypothetical protein	-
JG791_RS27180	10334..11302	-	969	WP_011251287.1	IS5 family transposase	-
JG791_RS27185	11449..11868	-	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
JG791_RS27190	11865..12176	-	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
JG791_RS27195	12381..12818	-	438	Protein_14	DDE-type integrase/transposase/recombinase	-
JG791_RS27200	12953..13650	+	698	WP_223175001.1	IS1-like element IS1A family transposase	-
JG791_RS27205	13652..14101	+	450	WP_011251283.1	thioredoxin fold domain-containing protein	-
JG791_RS27210	14118..14429	+	312	WP_011251282.1	hypothetical protein	-
JG791_RS27215	14443..14784	+	342	WP_011251281.1	hypothetical protein	-
JG791_RS27220	14844..15800	+	957	WP_011251280.1	DsbA family protein	-
JG791_RS27225	16225..16665	-	441	WP_011251275.1	hypothetical protein	-
JG791_RS27230	16671..17165	-	495	WP_011251274.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..221472	221472
	-	inside	IScluster/Tn	-	-	1..19085	19084

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T185383 WP_011251285.1 NZ_CP066905:c12176-11865 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T185383 NZ_CP088156:c7079872-7079684 [Bradyrhizobium sp. A19]
ATGAACAGTCGGGACGTCATCTCTGCTCTCCAGCGGGACGGATGGTTTCAGGTGGCTCAGAAGGGCAGCCACGTCCAGTT
CAAGCATCTCAGTAAGAGGGGCCGCGTAACCGTTCCTCATCCCGAACGGGACCTTCCGATCGGCACACTGAAGAGCATCG
AGAAGCAGTCAGGGTTGAAACTGAGGTGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT185383 WP_011251286.1 NZ_CP066905:c11868-11449 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT185383 NZ_CP088156:c7079678-7079277 [Bradyrhizobium sp. A19]
ATGCGGCATTACATCGGTCTGATCCATAAGGATGCTGACAGCCATTTCGGGGTCTCGTTTCCCGATCTGCCGGGCGTCGT
GACCGCCGGCACCAGCCTGGATGACGCGCGCATGATGGCCGAAGAGGCCTTGGCTTTGCATATTGAAGGCCTGATCGAGG
ACGGTGAAGCGATTCCCGAACCGTCGTCGCTGGAGCAGATCATGTCCGATCGGGACAACCGCTCCGGCGTCGCCGTTCTC
GTCCCGGCGAAGATCGATCAGCCGAAAGCGGTCCGCGTCAACGTCACGCTGCCGGAGGAAGTGCTGGCTCAGATCGATCA
GTATGCAGAGGCTCACGGCTTCACCCGATCCGGATTGCTTGTCCAGGCCGCCAAAAGGCTGATGACCGACGAGGCGGCCT
GA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1