Detailed information of TA system TA185361

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 135816..136085
• Replicon: plasmid pXHKP309-1
• Accession: NZ_CP066901
• Organism: Klebsiella pneumoniae strain XHKP309

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: JG788_RS27700
• Protein ID: WP_001372321.1
• Coordinates: 135960..136085 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	135816..135881 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JG788_RS27670	131526..132053	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
JG788_RS27675	132111..132344	+	234	WP_000006003.1	DUF905 family protein	-
JG788_RS27680	132405..134428	+	2024	Protein_180	ParB/RepB/Spo0J family partition protein	-
JG788_RS27685	134497..134931	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
JG788_RS27690	134928..135647	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	135659..135883	+	225	NuclAT_0	-	-
-	135659..135883	+	225	NuclAT_0	-	-
-	135659..135883	+	225	NuclAT_0	-	-
-	135659..135883	+	225	NuclAT_0	-	-
-	135816..135881	-	66	-	-	Antitoxin
JG788_RS27695	135869..136018	+	150	Protein_183	plasmid maintenance protein Mok	-
JG788_RS27700	135960..136085	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
JG788_RS27705	136404..136700	-	297	Protein_185	hypothetical protein	-
JG788_RS27710	137000..137296	+	297	WP_001272251.1	hypothetical protein	-
JG788_RS27715	137407..138228	+	822	WP_001234445.1	DUF932 domain-containing protein	-
JG788_RS27720	138525..139172	-	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
JG788_RS27725	139449..139832	+	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
JG788_RS27730	140023..140709	+	687	WP_015059009.1	PAS domain-containing protein	-
JG788_RS27735	140803..141030	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaKPC-2 / catA2 / mph(A) / blaTEM-1B / rmtB / fosA3 / blaCTX-M-55	-	1..178237	178237

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T185361 WP_001372321.1 NZ_CP066901:135960-136085 [Klebsiella pneumoniae]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T185361 NZ_CP088145:c716335-715916 [Corynebacterium diphtheriae]
ATGGTGAACAAGTTGGTCTACTTATCCTGCACCTTAAATCAGGAGTGCGGGAAGCGAACAGGTCCTCACAGAGAACCTGA
ACCTTTTCTTGGATTCTCCGTGAGGGATCGGAGTGGGCTAGAGGGCGCGATCGCTTCAGTATTTCAATCGTCGTTTGGCG
TCTTGCTGTACCCGACCATCTACGACCAAGCGGCCGCACTCCTGTTCAAGCTGGCAAACGGGCATTACTTCCAAGACGGA
AACAAGCGAACAGCATTCCACCTGTGCTTAGCCTACCTACGGGCCAATAACGTTCACGTCAGTCCCCCTCGGTCTGATGA
CGTCATCACTTTACTTCAGACCACGGTGAGTCGCTCAGGAAGCCAAGAAAACGGGATAGCGTTCGTTAGACAAAAGCTTT
TGGACTGGACCGTAGACTGA

Antitoxin

Nucleotide

• Length: 66 bp

>AT185361 NZ_CP066901:c135881-135816 [Klebsiella pneumoniae]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin