Detailed information of TA system TA185358

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 92925..93178
• Replicon: plasmid pXHKP309-1
• Accession: NZ_CP066901
• Organism: Klebsiella pneumoniae strain XHKP309

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: JG788_RS27425
• Protein ID: WP_001312851.1
• Coordinates: 93029..93178 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	92925..92984 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JG788_RS27385 (88585)	88585..88650	-	66	Protein_121	helix-turn-helix domain-containing protein	-
JG788_RS27390 (88703)	88703..89407	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
JG788_RS27395 (89432)	89432..89632	+	201	WP_072354025.1	hypothetical protein	-
JG788_RS27400 (89652)	89652..90398	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
JG788_RS27405 (90453)	90453..91013	+	561	WP_000139328.1	fertility inhibition protein FinO	-
JG788_RS27410 (91145)	91145..91345	+	201	WP_015059022.1	hypothetical protein	-
JG788_RS27415 (91731)	91731..92330	+	600	WP_032083981.1	PIN domain-containing protein	-
JG788_RS27420 (92392)	92392..92724	+	333	WP_152916585.1	hypothetical protein	-
- (92925)	92925..92984	-	60	NuclAT_1	-	Antitoxin
- (92925)	92925..92984	-	60	NuclAT_1	-	Antitoxin
- (92925)	92925..92984	-	60	NuclAT_1	-	Antitoxin
- (92925)	92925..92984	-	60	NuclAT_1	-	Antitoxin
JG788_RS27425 (93029)	93029..93178	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
JG788_RS27430 (93462)	93462..93710	+	249	WP_000083837.1	replication regulatory protein RepA	-
JG788_RS27435 (93825)	93825..94003	+	179	Protein_131	protein CopA/IncA	-
JG788_RS27440 (94022)	94022..94879	+	858	WP_032495102.1	incFII family plasmid replication initiator RepA	-
JG788_RS27445 (95818)	95818..96471	+	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
JG788_RS27450 (96564)	96564..96821	+	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
JG788_RS27455 (96754)	96754..97155	+	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
JG788_RS27460 (97404)	97404..97819	+	416	Protein_136	IS1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaKPC-2 / catA2 / mph(A) / blaTEM-1B / rmtB / fosA3 / blaCTX-M-55	-	1..178237	178237

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T185358 WP_001312851.1 NZ_CP066901:93029-93178 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T185358 NZ_CP088143:c83573-83268 [Salmonella enterica subsp. enterica serovar Gallinarum]
ATGCAGTTTAAGGTTTACACCTGTAAAAGGGAGAGTCGCTACCGGCTGTTTGTGGACGTGCAGAGCGACATCATTGACAC
CCCCGGACGGCGGATGGCTGTCCCGCTGGTCAGCGCCCGTCTGCTGTCGGAAAAGGTTCCCCGCGATCTTTACCCGGTGA
TGCATATCGGGGATGAGCCTTACCGCCTGCTGACCACGGATATGACCAGTGTGCCGGCCACCGTCATCGGGGAAGAGGTG
GCCGATCTCAGCCTCCGGGAGAACGATATCAAAAACGCCATTAACCTGATGTTCCGGGGGATCTGA

Antitoxin

Nucleotide

• Length: 60 bp

>AT185358 NZ_CP066901:c92984-92925 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin