Detailed information of TA system TA185337

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 162528..162797
• Replicon: plasmid pXHKP75-2
• Accession: NZ_CP066897
• Organism: Klebsiella pneumoniae strain XHKP75

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: JG785_RS29265
• Protein ID: WP_001372321.1
• Coordinates: 162672..162797 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	162528..162593 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JG785_RS29235	158238..158765	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
JG785_RS29240	158823..159056	+	234	WP_000006003.1	DUF905 family protein	-
JG785_RS29245	159117..161140	+	2024	Protein_211	ParB/RepB/Spo0J family partition protein	-
JG785_RS29250	161209..161643	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
JG785_RS29255	161640..162359	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	162371..162595	+	225	NuclAT_0	-	-
-	162371..162595	+	225	NuclAT_0	-	-
-	162371..162595	+	225	NuclAT_0	-	-
-	162371..162595	+	225	NuclAT_0	-	-
-	162528..162593	-	66	-	-	Antitoxin
JG785_RS29260	162581..162730	+	150	Protein_214	plasmid maintenance protein Mok	-
JG785_RS29265	162672..162797	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
JG785_RS29270	163116..163412	-	297	Protein_216	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaKPC-2 / blaCTX-M-65 / blaTEM-1B / rmtB	-	1..163729	163729

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T185337 WP_001372321.1 NZ_CP066897:162672-162797 [Klebsiella pneumoniae]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T185337 NZ_CP088138:c4379064-4378573 [Salmonella enterica subsp. enterica serovar Hissar]
ATGATCTCCACCCCTGAGCCGCTTCATGCCGGACATATTCTTACTCCGTTTTGCTGCGGTGTGGATTCTATAGATAACTG
GCTGGAACAGCGGGCGATGAAAAATCAGACCACTGGCGCGTCCCGTACCTTTGTGTGTTGTGGCAGCGATTCGAACGTAC
TGGCCTATTACTCGCTGGCGTCCAGCGCGGTCACGACGAATACCTCCCCCGGTCGCTTTCGTCGCAATATGCCTGACCCG
ATTCCGGTTGTGGTATTGGGGCGTCTGGCGGTGGATAAATCGCTTCATGGGCAGGGCGTCGCTCGGGCGCTGGTACGCGA
CGCAGGGCTGCGGGTTATTCAGGTGGCGGAGACTATCGGCATTCGTGGGATGCTGGTTCACGCCCTGTCGGATGAAGCGC
GGGAATTTTATCAGCGGGTGGGGTTTGTACCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGGGGGATTTGGTG
GAGAGTGTTTAA

Antitoxin

Nucleotide

• Length: 66 bp

>AT185337 NZ_CP066897:c162593-162528 [Klebsiella pneumoniae]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin