Detailed information of TA system TA185334

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 126383..126636
• Replicon: plasmid pXHKP75-2
• Accession: NZ_CP066897
• Organism: Klebsiella pneumoniae strain XHKP75

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: JG785_RS29020
• Protein ID: WP_001312851.1
• Coordinates: 126487..126636 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	126383..126442 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JG785_RS28980 (122043)	122043..122108	-	66	Protein_158	helix-turn-helix domain-containing protein	-
JG785_RS28985 (122161)	122161..122865	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
JG785_RS28990 (122890)	122890..123090	+	201	WP_072354025.1	hypothetical protein	-
JG785_RS28995 (123110)	123110..123856	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
JG785_RS29000 (123911)	123911..124471	+	561	WP_000139328.1	fertility inhibition protein FinO	-
JG785_RS29005 (124603)	124603..124803	+	201	WP_015059022.1	hypothetical protein	-
JG785_RS29010 (125189)	125189..125788	+	600	WP_032083981.1	PIN domain-containing protein	-
JG785_RS29015 (125850)	125850..126182	+	333	WP_152916585.1	hypothetical protein	-
- (126383)	126383..126442	-	60	NuclAT_1	-	Antitoxin
- (126383)	126383..126442	-	60	NuclAT_1	-	Antitoxin
- (126383)	126383..126442	-	60	NuclAT_1	-	Antitoxin
- (126383)	126383..126442	-	60	NuclAT_1	-	Antitoxin
JG785_RS29020 (126487)	126487..126636	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
JG785_RS29025 (126920)	126920..127168	+	249	WP_000083837.1	replication regulatory protein RepA	-
JG785_RS29030 (127283)	127283..127461	+	179	Protein_168	protein CopA/IncA	-
JG785_RS29035 (127480)	127480..128337	+	858	WP_032495102.1	incFII family plasmid replication initiator RepA	-
JG785_RS29040 (129276)	129276..129929	+	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
JG785_RS29045 (130022)	130022..130279	+	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
JG785_RS29050 (130212)	130212..130613	+	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
JG785_RS29055 (130862)	130862..131277	+	416	Protein_173	IS1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaKPC-2 / blaCTX-M-65 / blaTEM-1B / rmtB	-	1..163729	163729

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T185334 WP_001312851.1 NZ_CP066897:126487-126636 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T185334 NZ_CP088138:3744914-3745189 [Salmonella enterica subsp. enterica serovar Hissar]
ATGAATGCCTCCGGCGTCAGCGTCCGCCATATCAACAGCGAAACCTGCATGACCGCCTGTTACTCACAAATCCCCAGCCA
GCACCTTAAGGGTGACTGGCAGGAAGAAGCGGGATTTGAGACCGGGCACGGCGTCACCGTGAAGATCTCCGAGGGGTGTT
TGATCCTGATTGCAGAGACGGACGAAGTGAGGGATCTACGGAAGGAGCTTTATCAGGTGAAAAAGTCGATGAAGCATATT
AAGGCGGGGGTAAATAATGTGGTGAACGGGGATTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT185334 NZ_CP066897:c126442-126383 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin