Detailed information of TA system TA185307

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 83634..83887
• Replicon: plasmid pXHKP53-1
• Accession: NZ_CP066892
• Organism: Klebsiella pneumoniae strain XHKP53

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: JG783_RS27195
• Protein ID: WP_001312851.1
• Coordinates: 83634..83783 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	83828..83887 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JG783_RS27160 (78994)	78994..79409	-	416	Protein_101	IS1 family transposase	-
JG783_RS27165 (79658)	79658..80059	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
JG783_RS27170 (79992)	79992..80249	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
JG783_RS27175 (80342)	80342..80995	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
JG783_RS27180 (81933)	81933..82790	-	858	WP_032495102.1	incFII family plasmid replication initiator RepA	-
JG783_RS27185 (82809)	82809..82987	-	179	Protein_106	protein CopA/IncA	-
JG783_RS27190 (83102)	83102..83350	-	249	WP_000083837.1	replication regulatory protein RepA	-
JG783_RS27195 (83634)	83634..83783	-	150	WP_001312851.1	Hok/Gef family protein	Toxin
- (83828)	83828..83887	+	60	NuclAT_1	-	Antitoxin
- (83828)	83828..83887	+	60	NuclAT_1	-	Antitoxin
- (83828)	83828..83887	+	60	NuclAT_1	-	Antitoxin
- (83828)	83828..83887	+	60	NuclAT_1	-	Antitoxin
JG783_RS27200 (84087)	84087..84419	-	333	WP_152916585.1	hypothetical protein	-
JG783_RS27205 (84481)	84481..85080	-	600	WP_032083981.1	PIN domain-containing protein	-
JG783_RS27210 (85466)	85466..85666	-	201	WP_015059022.1	hypothetical protein	-
JG783_RS27215 (85798)	85798..86358	-	561	WP_000139328.1	fertility inhibition protein FinO	-
JG783_RS27220 (86413)	86413..87159	-	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
JG783_RS27225 (87179)	87179..87379	-	201	WP_072354025.1	hypothetical protein	-
JG783_RS27230 (87404)	87404..88108	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
JG783_RS27235 (88161)	88161..88226	+	66	Protein_116	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaKPC-2 / blaSHV-12 / rmtB / blaTEM-1B / fosA3 / blaCTX-M-65 / catA2	-	1..147717	147717

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T185307 WP_001312851.1 NZ_CP066892:c83783-83634 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T185307 NZ_CP088135:c13636-13331 [Salmonella enterica subsp. enterica serovar Gallinarum]
ATGCAGTTTAAGGTTTACACCTGTAAAAGGGAGAGTCGCTACCGGCTGTTTGTGGACGTGCAGAGCGACATCATTGACAC
CCCCGAACGGCGGATGGCTGTCCCGCTGGTCAGCGCCCGTCTGCTGTCGGAAAAGGTTCCCCGCGATCTTTACCCGGTGA
TGCATATCGGGGATGAGCCTTACCGCCTGCTGACCACGGATATGACCAGTGTGCCGGCCACCGTCATCGGGGAAGAGGTG
GCCGATCTCAGCCTCCGGGAGAACGATATCAAAAACGCCATTAACCTGATGTTCCGGGGGATCTGA

Antitoxin

Nucleotide

• Length: 60 bp

>AT185307 NZ_CP066892:83828-83887 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin