Detailed information of TA system TA185304

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 45388..45814
• Replicon: plasmid pXHKP53-1
• Accession: NZ_CP066892
• Organism: Klebsiella pneumoniae strain XHKP53

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: JG783_RS26935
• Protein ID: WP_001372321.1
• Coordinates: 45388..45513 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	45590..45814 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JG783_RS26905 (40692)	40692..41396	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
JG783_RS26910 (41644)	41644..42027	-	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
JG783_RS26915 (42359)	42359..42949	+	591	WP_000252683.1	transglycosylase SLT domain-containing protein	-
JG783_RS26920 (43246)	43246..44067	-	822	WP_001234445.1	DUF932 domain-containing protein	-
JG783_RS26925 (44178)	44178..44474	-	297	WP_001272251.1	hypothetical protein	-
JG783_RS26930 (44774)	44774..45069	+	296	Protein_55	hypothetical protein	-
JG783_RS26935 (45388)	45388..45513	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
JG783_RS26940 (45455)	45455..45604	-	150	Protein_57	plasmid maintenance protein Mok	-
- (45590)	45590..45814	-	225	NuclAT_0	-	Antitoxin
- (45590)	45590..45814	-	225	NuclAT_0	-	Antitoxin
- (45590)	45590..45814	-	225	NuclAT_0	-	Antitoxin
- (45590)	45590..45814	-	225	NuclAT_0	-	Antitoxin
JG783_RS26945 (45826)	45826..46545	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
JG783_RS26950 (46542)	46542..46976	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
JG783_RS26955 (47045)	47045..49068	-	2024	Protein_60	ParB/RepB/Spo0J family partition protein	-
JG783_RS26960 (49129)	49129..49362	-	234	WP_000006003.1	DUF905 family protein	-
JG783_RS26965 (49420)	49420..49946	-	527	Protein_62	single-stranded DNA-binding protein	-
JG783_RS26970 (50247)	50247..50702	+	456	Protein_63	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaKPC-2 / blaSHV-12 / rmtB / blaTEM-1B / fosA3 / blaCTX-M-65 / catA2	-	1..147717	147717

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T185304 WP_001372321.1 NZ_CP066892:c45513-45388 [Klebsiella pneumoniae]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T185304 NZ_CP088134:c2968035-2967622 [Salmonella enterica subsp. enterica serovar Gallinarum]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCTTTATGGATGATACTACTGTCACTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGCCAGGGAGAGATCAAGCTACTCATGGACGGGCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTCTTGCGTCCGCCCTGGTTACTGAAGAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
CAGGCCAACACTGA

Antitoxin

Nucleotide

• Length: 225 bp

>AT185304 NZ_CP066892:c45814-45590 [Klebsiella pneumoniae]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin