Detailed information of TA system TA185281

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 166857..167126
• Replicon: plasmid pXHKP6-1
• Accession: NZ_CP066888
• Organism: Klebsiella pneumoniae strain XHKP6

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: JG758_RS27955
• Protein ID: WP_001372321.1
• Coordinates: 167001..167126 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	166857..166922 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JG758_RS27925	162567..163094	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
JG758_RS27930	163152..163385	+	234	WP_000006003.1	DUF905 family protein	-
JG758_RS27935	163446..165469	+	2024	Protein_215	ParB/RepB/Spo0J family partition protein	-
JG758_RS27940	165538..165972	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
JG758_RS27945	165969..166688	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	166700..166924	+	225	NuclAT_0	-	-
-	166700..166924	+	225	NuclAT_0	-	-
-	166700..166924	+	225	NuclAT_0	-	-
-	166700..166924	+	225	NuclAT_0	-	-
-	166857..166922	-	66	-	-	Antitoxin
JG758_RS27950	166910..167059	+	150	Protein_218	plasmid maintenance protein Mok	-
JG758_RS27955	167001..167126	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
JG758_RS27960	167445..167741	-	297	Protein_220	hypothetical protein	-
JG758_RS27965	168041..168337	+	297	WP_001272251.1	hypothetical protein	-
JG758_RS27970	168448..169269	+	822	WP_001234445.1	DUF932 domain-containing protein	-
JG758_RS27975	169566..170213	-	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
JG758_RS27980	170490..170873	+	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
JG758_RS27985	171064..171750	+	687	WP_015059009.1	PAS domain-containing protein	-
JG758_RS27990	171844..172071	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaSHV-12 / blaKPC-2 / catA2 / blaCTX-M-65 / fosA3 / blaTEM-1B / rmtB	-	1..180300	180300

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T185281 WP_001372321.1 NZ_CP066888:167001-167126 [Klebsiella pneumoniae]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T185281 NZ_CP088131:1042400-1042735 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT185281 NZ_CP066888:c166922-166857 [Klebsiella pneumoniae]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin