Detailed information of TA system TA185278

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 128623..128876
• Replicon: plasmid pXHKP6-1
• Accession: NZ_CP066888
• Organism: Klebsiella pneumoniae strain XHKP6

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: JG758_RS27695
• Protein ID: WP_001312851.1
• Coordinates: 128727..128876 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	128623..128682 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JG758_RS27655 (124283)	124283..124348	-	66	Protein_159	helix-turn-helix domain-containing protein	-
JG758_RS27660 (124401)	124401..125105	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
JG758_RS27665 (125130)	125130..125330	+	201	WP_072354025.1	hypothetical protein	-
JG758_RS27670 (125350)	125350..126096	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
JG758_RS27675 (126151)	126151..126711	+	561	WP_000139328.1	fertility inhibition protein FinO	-
JG758_RS27680 (126843)	126843..127043	+	201	WP_015059022.1	hypothetical protein	-
JG758_RS27685 (127429)	127429..128028	+	600	WP_032083981.1	PIN domain-containing protein	-
JG758_RS27690 (128090)	128090..128422	+	333	WP_152916585.1	hypothetical protein	-
- (128623)	128623..128682	-	60	NuclAT_1	-	Antitoxin
- (128623)	128623..128682	-	60	NuclAT_1	-	Antitoxin
- (128623)	128623..128682	-	60	NuclAT_1	-	Antitoxin
- (128623)	128623..128682	-	60	NuclAT_1	-	Antitoxin
JG758_RS27695 (128727)	128727..128876	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
JG758_RS27700 (129160)	129160..129408	+	249	WP_000083837.1	replication regulatory protein RepA	-
JG758_RS27705 (129523)	129523..129701	+	179	Protein_169	protein CopA/IncA	-
JG758_RS27710 (129720)	129720..130577	+	858	WP_032495102.1	incFII family plasmid replication initiator RepA	-
JG758_RS27715 (131516)	131516..132169	+	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
JG758_RS27720 (132262)	132262..132519	+	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
JG758_RS27725 (132452)	132452..132853	+	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
JG758_RS27730 (133102)	133102..133517	+	416	Protein_174	IS1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaSHV-12 / blaKPC-2 / catA2 / blaCTX-M-65 / fosA3 / blaTEM-1B / rmtB	-	1..180300	180300

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T185278 WP_001312851.1 NZ_CP066888:128727-128876 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T185278 NZ_CP088131:664706-665020 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCCTAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Nucleotide

• Length: 60 bp

>AT185278 NZ_CP066888:c128682-128623 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin