Detailed information of TA system TA185248

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4564064..4564666
• Replicon: chromosome
• Accession: NZ_CP066851
• Organism: Salmonella enterica subsp. enterica serovar Heidelberg strain SH-2813-Parental

Toxin (Protein)

• Gene name: higB
• Uniprot ID: M7S4R6
• Locus tag: JIG31_RS22040
• Protein ID: WP_001159635.1
• Coordinates: 4564355..4564666 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JIG31_RS22035
• Protein ID: WP_000362050.1
• Coordinates: 4564064..4564354 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JIG31_RS22020 (4561557)	4561557..4562459	+	903	WP_000331361.1	formate dehydrogenase subunit beta	-
JIG31_RS22025 (4562456)	4562456..4563091	+	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
JIG31_RS22030 (4563088)	4563088..4564017	+	930	WP_000027730.1	formate dehydrogenase accessory protein FdhE	-
JIG31_RS22035 (4564064)	4564064..4564354	-	291	WP_000362050.1	DNA-binding transcriptional regulator	Antitoxin
JIG31_RS22040 (4564355)	4564355..4564666	-	312	WP_001159635.1	cytotoxic translational repressor of toxin-antitoxin stability system	Toxin
JIG31_RS22045 (4564884)	4564884..4565813	+	930	WP_001127705.1	alpha/beta hydrolase	-
JIG31_RS22050 (4565899)	4565899..4566210	+	312	WP_000558163.1	type II toxin-antitoxin system HigB family toxin	-
JIG31_RS22055 (4566207)	4566207..4566653	+	447	WP_001259012.1	type II toxin-antitoxin system HigA family antitoxin	-
JIG31_RS22060 (4566668)	4566668..4567609	-	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
JIG31_RS22065 (4567654)	4567654..4568091	-	438	WP_000560969.1	D-aminoacyl-tRNA deacylase	-
JIG31_RS22070 (4568088)	4568088..4568960	-	873	WP_000921423.1	virulence factor BrkB family protein	-
JIG31_RS22075 (4568954)	4568954..4569553	-	600	WP_000965702.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12340.30 Da
• Isoelectric Point: 9.4460

>T185248 WP_001159635.1 NZ_CP066851:c4564666-4564355 [Salmonella enterica subsp. enterica serovar Heidelberg]
MQFIETELFTEDVKKLLDEDEYHKLQVFMAQHPDCGDVIQETGGLRKMRWGARGKGKRSGVRIIYFHRSQRYEIRLLLIY
QKGIKDDLTPQEKAVLRMLNERW

Nucleotide

• Length: 312 bp

>T185248 NZ_CP088120:c5174668-5174285 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10971.59 Da
• Isoelectric Point: 10.6525

>AT185248 WP_000362050.1 NZ_CP066851:c4564354-4564064 [Salmonella enterica subsp. enterica serovar Heidelberg]
MDKVLFERLTQSMSQMNEIIEGTREPSRTFHIDAMKIKEIRQASGLSQSKFAELISVNVDTLRNWEQGRRSPTGPAKALL
RAIANDPRNVIQALRY

Nucleotide

• Length: 291 bp

>AT185248 NZ_CP088120:c5174910-5174668 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• AlphaFold DB: A0A3V2JK97