Detailed information of TA system TA185223

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 78320..78741
• Replicon: plasmid pZ30-TEM-1B
• Accession: NZ_CP066845
• Organism: Escherichia coli strain Z30

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: JHL13_RS23145
• Protein ID: WP_096937776.1
• Coordinates: 78320..78445 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	78543..78741 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JHL13_RS23110 (73387)	73387..73602	-	216	WP_001352842.1	conjugal transfer relaxosome protein TraY	-
JHL13_RS23115 (73738)	73738..74385	-	648	WP_000332520.1	conjugal transfer transcriptional regulator TraJ	-
JHL13_RS23120 (74577)	74577..74960	-	384	WP_072647915.1	conjugal transfer relaxosome DNA-binding protein TraM	-
JHL13_RS23125 (75312)	75312..75902	+	591	WP_166494936.1	transglycosylase SLT domain-containing protein	-
JHL13_RS23130 (76197)	76197..77018	-	822	WP_001234475.1	DUF932 domain-containing protein	-
JHL13_RS23135 (77137)	77137..77424	-	288	WP_000107546.1	hypothetical protein	-
JHL13_RS23140 (77741)	77741..78019	+	279	Protein_84	hypothetical protein	-
JHL13_RS23145 (78320)	78320..78445	-	126	WP_096937776.1	type I toxin-antitoxin system Hok family toxin	Toxin
JHL13_RS23150 (78387)	78387..78536	-	150	Protein_86	DUF5431 family protein	-
- (78543)	78543..78741	-	199	NuclAT_0	-	Antitoxin
- (78543)	78543..78741	-	199	NuclAT_0	-	Antitoxin
- (78543)	78543..78741	-	199	NuclAT_0	-	Antitoxin
- (78543)	78543..78741	-	199	NuclAT_0	-	Antitoxin
JHL13_RS23155 (78710)	78710..79472	-	763	Protein_87	plasmid SOS inhibition protein A	-
JHL13_RS23160 (79469)	79469..79903	-	435	WP_000845928.1	conjugation system SOS inhibitor PsiB	-
JHL13_RS23165 (79958)	79958..81916	-	1959	WP_029487628.1	ParB/RepB/Spo0J family partition protein	-
JHL13_RS23170 (81982)	81982..82215	-	234	WP_000005990.1	DUF905 family protein	-
JHL13_RS23175 (82272)	82272..82811	-	540	WP_000290801.1	single-stranded DNA-binding protein	-
JHL13_RS23180 (83129)	83129..83638	+	510	WP_071600123.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / dfrA14 / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / sitABCD	vat / iutA / iucD / iucC / iucB / iucA	1..127534	127534

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4806.72 Da
• Isoelectric Point: 8.4890

>T185223 WP_096937776.1 NZ_CP066845:c78445-78320 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T185223 NZ_CP088115:c681324-680905 [Sinorhizobium meliloti]
GTGAGCAGGCTCTACATGCTGGACACCAATATCGTGTCCGAGCTTGCGCGAAACCCACAGGGGGCTGTCACTAAACGCAT
TGCCGAGGTAGGACCGGATGCAATCTGCGTCAGCATCATTACGGCGGCAGAGTTACGCTATGGATGCGCCAAGAAGGGAT
CATCGAAACTACTGGCGCAGATCGAGACCATTCTTGGAAGTATGCAAGTGCTTGCGCTCGATGTGCCTGCTGATGCCGAA
TACGGCGGCATCCGGGCTGAACTGGAGGCCGCTGGTAAGCCCATCGGCCCGAATGACCTGTTCATCGCCGCTCATGCCTG
TATGCTTGGAGCGGTGCTGGTAACGGCCAACAGTAGCGAGTTCACACGCGTTCGTGATCTCAAGGTCGAGAACTGGCTGG
ATTTCACTCCTTCGGGTTGA

Antitoxin

Nucleotide

• Length: 199 bp

>AT185223 NZ_CP066845:c78741-78543 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin