Detailed information of TA system TA185220

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 40168..40422
• Replicon: plasmid pZ30-TEM-1B
• Accession: NZ_CP066845
• Organism: Escherichia coli strain Z30

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: JHL13_RS22920
• Protein ID: WP_001312851.1
• Coordinates: 40168..40317 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	40361..40422 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JHL13_RS22890 (35450)	35450..38455	+	3006	WP_001143760.1	Tn3-like element Tn3 family transposase	-
JHL13_RS22895 (38388)	38388..38853	-	466	Protein_35	plasmid replication initiator RepA	-
JHL13_RS22900 (38846)	38846..39328	-	483	WP_001273588.1	hypothetical protein	-
JHL13_RS22905 (39321)	39321..39368	-	48	WP_229471593.1	hypothetical protein	-
JHL13_RS22910 (39359)	39359..39610	+	252	WP_223195197.1	replication protein RepA	-
JHL13_RS22915 (39627)	39627..39884	-	258	WP_000083845.1	replication regulatory protein RepA	-
JHL13_RS22920 (40168)	40168..40317	-	150	WP_001312851.1	Hok/Gef family protein	Toxin
- (40361)	40361..40422	+	62	NuclAT_1	-	Antitoxin
- (40361)	40361..40422	+	62	NuclAT_1	-	Antitoxin
- (40361)	40361..40422	+	62	NuclAT_1	-	Antitoxin
- (40361)	40361..40422	+	62	NuclAT_1	-	Antitoxin
JHL13_RS22925 (40678)	40678..40752	-	75	Protein_41	endonuclease	-
JHL13_RS22930 (40998)	40998..41210	-	213	WP_001299730.1	ANR family transcriptional regulator	-
JHL13_RS22935 (41346)	41346..41906	-	561	WP_000139315.1	fertility inhibition protein FinO	-
JHL13_RS22940 (42009)	42009..42869	-	861	WP_000704513.1	alpha/beta hydrolase	-
JHL13_RS22945 (42928)	42928..43674	-	747	WP_000205749.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / dfrA14 / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / sitABCD	vat / iutA / iucD / iucC / iucB / iucA	1..127534	127534

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T185220 WP_001312851.1 NZ_CP066845:c40317-40168 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T185220 NZ_CP088114:1234922-1235365 [Sinorhizobium meliloti]
GTGACTTTCCTGCTCGACGTCAATGTGCTGATTGCCTTGATCGATCCGAGCCATATAGGCCATGATGATGCGCACGAATG
GTTCGCGTCGATTGGTCAGACCGCGTGGGCCACCTGCCCAATTACCGAAAATGGTGTCATCCGGATCGTCGGCAATCCCG
AGTATCCCAATTCTCCCGGCTCACCGTTACCCGCGATGGAGATCGTCGCCAAATTGCGGTCGCTCCCCGGTCATGTTTTT
TGGCCGGACGATGTGAGCCTTGTCGGGTCAAGTGATATCATCCCATCGAAAATCCTGACTTCGGGGCAGGTGACTGACAC
CTATCTGCTTGCGCTCGCCAAGGCGCGTGGGGGCAAGCTGGCGACATTCGATCGCAAGCTGTCGGCAGCGGCGGTCACGA
AGGGCAATTCCGCCTTGCACCTGATCGCGACGAATAGGTCGTGA

Antitoxin

Nucleotide

• Length: 62 bp

>AT185220 NZ_CP066845:40361-40422 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin