Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3683452..3684146 Replicon chromosome
Accession NZ_CP066844
Organism Escherichia coli strain Z30

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag JHL13_RS17470 Protein ID WP_001263491.1
Coordinates 3683452..3683850 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag JHL13_RS17475 Protein ID WP_000554755.1
Coordinates 3683853..3684146 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JHL13_RS17440 (3678714) 3678714..3680171 + 1458 WP_022645227.1 cytosol nonspecific dipeptidase -
JHL13_RS17445 (3680180) 3680180..3680461 + 282 WP_022645226.1 hypothetical protein -
JHL13_RS17450 (3680478) 3680478..3680987 - 510 WP_001361775.1 metal-dependent hydrolase -
JHL13_RS17455 (3681049) 3681049..3681663 - 615 WP_022645225.1 peptide chain release factor H -
JHL13_RS17460 (3681660) 3681660..3682799 - 1140 WP_022645224.1 RNA ligase RtcB family protein -
JHL13_RS17465 (3682990) 3682990..3683442 - 453 WP_023909048.1 GNAT family N-acetyltransferase -
JHL13_RS17470 (3683452) 3683452..3683850 - 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
JHL13_RS17475 (3683853) 3683853..3684146 - 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
JHL13_RS17480 (3684198) 3684198..3685253 - 1056 WP_022645222.1 DNA polymerase IV -
JHL13_RS17485 (3685324) 3685324..3686109 - 786 WP_000207551.1 putative lateral flagellar export/assembly protein LafU -
JHL13_RS17490 (3686081) 3686081..3687793 + 1713 Protein_3429 flagellar biosynthesis protein FlhA -
JHL13_RS17495 (3687891) 3687891..3688664 - 774 WP_022645219.1 C40 family peptidase -
JHL13_RS17500 (3688850) 3688850..3689110 + 261 WP_000729708.1 type II toxin-antitoxin system antitoxin DinJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T185216 WP_001263491.1 NZ_CP066844:c3683850-3683452 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T185216 NZ_CP088113:2509326-2509808 [Sinorhizobium meliloti]
ATGCTGTCGGCGCCGACGCCTCTTGGTGAAGCCCACGACTTCGATCTCTTCCAGAGCGGCAACGCCACGCTCGATGACTG
GCTGCGCCGCCGTGCGCACGCTAATCAGGCCAGTGGCGCGTCGCGCACCTATGTTATCGCTGAAGAATGGCGCGTGGTCG
GCTATTACTGCCTTGCATCCGGTGCCCTCGATCTGGCCGACGCGCCGAGCTCTGTCCGGCGCAACATGCCGGATCCCATC
CCGATGGCGGTGCTCGGTCGCCTTGCTATCGACCGCGACTGGCAGGGCAAAGGGTTGGGCGCGGCGCTATTGCAAGATGC
CGTCCTGCGGTCCTCGCAGGCGGCCGACATCATGGGAATACGGGGCTTGCTCGTGCATGCCATCTCGGGCGAGGCGAAAG
CCTTTTACGAACATTACGGCTTCCAGTGCTCACCCAACCATCCCATGACGCTGGTGTTGTCGCTCAAGGGCAAGCGCCGT
TAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT185216 WP_000554755.1 NZ_CP066844:c3684146-3683853 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT185216 NZ_CP088113:2509042-2509326 [Sinorhizobium meliloti]
ATGGCCACGAAATCGTCGACTGACACCCACGCGAAGCCCGTCAACCTGCGAATCCGAGAGGATATACGCAAGGTGATCGA
TCGCGCCGCGAGCATTCGCGGCAAGACCCGCTCCGATTTTATGATCGACGCAGCCTACCGCGCGGCCGAAGACACGCTGC
TCGATCAGACGCTGGTGCGGGTCGATGAGCACAGCTATCGGCACTACCTAGACATTCTTGACCAGCCGCCAAGTGGCGAC
GGCTTCGAACGCCTGATGAATGCGCCGAAACCCTGGCAGTCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References