Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 3459370..3460049 Replicon chromosome
Accession NZ_CP066844
Organism Escherichia coli strain Z30

Toxin (Protein)


Gene name higB Uniprot ID A0A0A1A1R1
Locus tag JHL13_RS16420 Protein ID WP_000057541.1
Coordinates 3459747..3460049 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag JHL13_RS16415 Protein ID WP_000806442.1
Coordinates 3459370..3459711 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JHL13_RS16405 (3455613) 3455613..3456545 - 933 WP_000883035.1 glutaminase A -
JHL13_RS16410 (3456808) 3456808..3459312 + 2505 WP_001601790.1 copper-exporting P-type ATPase CopA -
JHL13_RS16415 (3459370) 3459370..3459711 - 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
JHL13_RS16420 (3459747) 3459747..3460049 - 303 WP_000057541.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JHL13_RS16425 (3460182) 3460182..3460976 + 795 WP_000365164.1 TraB/GumN family protein -
JHL13_RS16430 (3461180) 3461180..3461659 + 480 WP_000186627.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
JHL13_RS16435 (3461696) 3461696..3463348 - 1653 WP_077516164.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
JHL13_RS16440 (3463566) 3463566..3464786 + 1221 WP_001251634.1 fosmidomycin MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11795.37 Da        Isoelectric Point: 10.2638

>T185214 WP_000057541.1 NZ_CP066844:c3460049-3459747 [Escherichia coli]
MAQKNNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

>T185214 NZ_CP088113:1845169-1845357 [Sinorhizobium meliloti]
ATGAAGAGCGGCGACATTATTGCAGCATTGCAGAAGGACGGATGGTACGAGGTTGCGACCAAGGGCAGCCACGTTCAATT
CAAACATCCGAAAAAGCACGGCCGGGTTACCGTTCCTCACCCGAAGAGGGATCTACCAATCGGTACCCTCAGGAGCATTG
AAAAGCAGTCCGGTTTGAAATTGAGGTAA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT185214 WP_000806442.1 NZ_CP066844:c3459711-3459370 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp

>AT185214 NZ_CP088113:1845362-1845799 [Sinorhizobium meliloti]
ATGCGCAACTATATCGGATTGATCCACAAGGACGCCGAGAGCGATTACGGCGTTTCCTTTCCTGATTTTTCCGGCGTCGT
GACCGCCGGTGCCGACCTCGACGACGCGCGCGCTATGGCAGAGGAAGCCTTGGCCTTGCATATTGAAGGGCTGGTAGAGG
ACGGCGAGGCCATCCCGGAGCCCTCCTCCCTTGAAGTTGTCATGTCCGACGTCGAGAACAGGGATTGCGTCGCTATTCTC
GTCGCGGTGAAAACCGAGGCAAAAAGAGCCATTCGAGTGAACGTTACGCTTCCCGAAGGTGTCCTGAAGCAGATAGATGC
CTTCGCCGAAGCCCACGGCCTTACGCGGTCTGGATTTCTTGCTCGTGCTGCAACGCACGAGATCGAACGGGCAAATGACG
GCCACGACGCCTATGCGGAATCCCGCTTGTCGGCTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A1A1R1

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References