Detailed information of TA system TA185198

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 632676..633475
• Replicon: chromosome
• Accession: NZ_CP066844
• Organism: Escherichia coli strain Z30

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: JHL13_RS03020
• Protein ID: WP_000347273.1
• Coordinates: 632676..633140 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: D6JF08
• Locus tag: JHL13_RS03025
• Protein ID: WP_001308975.1
• Coordinates: 633140..633475 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JHL13_RS02990 (627677)	627677..628111	-	435	WP_000948824.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-
JHL13_RS02995 (628129)	628129..629007	-	879	WP_001298314.1	PTS N-acetylgalactosamine transporter subunit IID	-
JHL13_RS03000 (628997)	628997..629776	-	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
JHL13_RS03005 (629787)	629787..630260	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
JHL13_RS03010 (630283)	630283..631563	-	1281	WP_023908831.1	tagatose-bisphosphate aldolase subunit KbaZ	-
JHL13_RS03015 (631812)	631812..632621	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
JHL13_RS03020 (632676)	632676..633140	-	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
JHL13_RS03025 (633140)	633140..633475	-	336	WP_001308975.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
JHL13_RS03030 (633624)	633624..635195	-	1572	WP_077516436.1	galactarate dehydratase	-
JHL13_RS03035 (635570)	635570..636904	+	1335	WP_000599651.1	galactarate/glucarate/glycerate transporter GarP	-
JHL13_RS03040 (636920)	636920..637690	+	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T185198 WP_000347273.1 NZ_CP066844:c633140-632676 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T185198 NZ_CP088102:c124075-123707 [Bradyrhizobium japonicum]
GTGAAACTCGTCTGGTCGCGCTTCGCGTTGTTCGATCGCGACGGCATTTTCAGCTACATCGAGGCCGAGAATCCTCGTGC
CGCGGTCCATGTCGACGAACAGATTGCGAATGCCGCGCGGCGCCTGCTCGACTTTCCGGATAGCGGTCGGCCGGGGCGTG
TCGCTGGGACGCGCGAGTTGGTCATTCCGCGTACATCTTATGTCATCGAAATCCTCGTGCCGCCCGAAAGCTTGCCGATC
CTCGAATGGCTCGGCATCAAAACGTCAAAATCACCACTTATCGTACGCTCTTGCGTTGATCTTGAACAATTGGCCTGTCG
ACAGCCCGTTGCGTATGCCGTCACCGACACCAGACGCGGCCCGCCATAG

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12343.97 Da
• Isoelectric Point: 4.8616

>AT185198 WP_001308975.1 NZ_CP066844:c633475-633140 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT185198 NZ_CP088102:c124380-124072 [Bradyrhizobium japonicum]
ATGGCTTCGAATGCCTTGGTCCAAACCCGCATCGATGCGGACGTGAAGGAAAAGGCCACTGCGGTTCTGGAAAATATGGG
TCTCACGGTTTCCGACGTCGTGCGGATCCTGCTTACGCGCACGGCCAATGAAGGGATGCTTCCGTTGGAGCTCGTGAGCA
ACAGCCAAGCTTACGATACCTGGTTTCGTGAGAAGGTGCGCCAGGCGTTGGCCGACACGCGGCCTGATATCGATGATTCC
GAGGTCGAAGCGCATTTCGCACAACGCCGTGCCGCTGCGCTTCGAAAAGCGCCGGAGCAGGAACGGTGA

Structures

Toxin

• AlphaFold DB: V0SSH7

Antitoxin

• AlphaFold DB: D6JF08