Detailed information of TA system
Overview
TA module
| Type | I | Classification (family/domain) | hok-sok/- |
| Location | 85742..86168 | Replicon | plasmid pIncF4-A-B1 |
| Accession | NZ_CP066841 | ||
| Organism | Escherichia coli strain Ec-RL2-1X | ||
Toxin (Protein)
| Gene name | hok | Uniprot ID | - |
| Locus tag | JH656_RS24285 | Protein ID | WP_001372321.1 |
| Coordinates | 85742..85867 (-) | Length | 42 a.a. |
Antitoxin (RNA)
| Gene name | sok | ||
| Locus tag | - | ||
| Coordinates | 85944..86168 (-) |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| JH656_RS24240 (80790) | 80790..81017 | - | 228 | WP_001254385.1 | conjugal transfer relaxosome protein TraY | - |
| JH656_RS24245 (81111) | 81111..81797 | - | 687 | WP_000332493.1 | PAS domain-containing protein | - |
| JH656_RS24250 (81988) | 81988..82371 | - | 384 | WP_000124979.1 | conjugal transfer relaxosome DNA-binding protein TraM | - |
| JH656_RS24255 (82648) | 82648..83295 | + | 648 | WP_000615259.1 | transglycosylase SLT domain-containing protein | - |
| JH656_RS24260 (83592) | 83592..84413 | - | 822 | WP_001234469.1 | DUF932 domain-containing protein | - |
| JH656_RS24265 (84534) | 84534..84821 | - | 288 | WP_000107535.1 | hypothetical protein | - |
| JH656_RS24270 (84846) | 84846..85052 | - | 207 | WP_000547971.1 | hypothetical protein | - |
| JH656_RS24275 (85122) | 85122..85295 | + | 174 | Protein_94 | hypothetical protein | - |
| JH656_RS24280 (85293) | 85293..85523 | - | 231 | WP_001426396.1 | hypothetical protein | - |
| JH656_RS24285 (85742) | 85742..85867 | - | 126 | WP_001372321.1 | type I toxin-antitoxin system Hok family toxin | Toxin |
| JH656_RS24290 (85809) | 85809..85958 | - | 150 | Protein_97 | plasmid maintenance protein Mok | - |
| - (85946) | 85946..86011 | - | 66 | NuclAT_1 | - | - |
| - (85944) | 85944..86168 | + | 225 | NuclAT_0 | - | - |
| - (85944) | 85944..86168 | - | 225 | NuclAT_0 | - | Antitoxin |
| - (85944) | 85944..86168 | - | 225 | NuclAT_0 | - | Antitoxin |
| - (85944) | 85944..86168 | - | 225 | NuclAT_0 | - | Antitoxin |
| - (85944) | 85944..86168 | - | 225 | NuclAT_0 | - | Antitoxin |
| JH656_RS24295 (85980) | 85980..86168 | + | 189 | WP_001299721.1 | hypothetical protein | - |
| JH656_RS24300 (86137) | 86137..86899 | - | 763 | Protein_99 | plasmid SOS inhibition protein A | - |
| JH656_RS24305 (86896) | 86896..87330 | - | 435 | WP_000845873.1 | conjugation system SOS inhibitor PsiB | - |
| JH656_RS24310 (87385) | 87385..89343 | - | 1959 | WP_000117210.1 | ParB/RepB/Spo0J family partition protein | - |
| JH656_RS24315 (89408) | 89408..89641 | - | 234 | WP_000006030.1 | DUF905 family protein | - |
| JH656_RS24320 (89703) | 89703..90242 | - | 540 | WP_000290812.1 | single-stranded DNA-binding protein | - |
| JH656_RS24325 (90268) | 90268..90474 | - | 207 | WP_000547971.1 | hypothetical protein | - |
| JH656_RS24330 (90715) | 90715..90942 | - | 228 | WP_071594011.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | sitABCD | iroB / iroC / iroD / iroE / iroN | 1..113350 | 113350 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 42 a.a. Molecular weight: 4780.69 Da Isoelectric Point: 8.5110
>T185194 WP_001372321.1 NZ_CP066841:c85867-85742 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK
Download Length: 126 bp
>T185194 NZ_CP088098:c93191-92808 [Rhizobium leguminosarum bv. viciae]
GTGATACTGGCAGACACTTCTATATGGATCGATCATTTCCGCCATCTTGATGCAGAGCTTCGGACGATCATTGAGGACGA
TCGTCTACTCTGCCATCCGGCTGTGGCTGGCGAACTGGCGCTTGGCAGCCTTCGAGATCGCGGGCGCGTGATAGCGTTTC
TGGCGGCCCAGCGCCAAGCGTTCGTCGCAACGCACGACGAAGTGATGACAATGATTGATCGTCACGGCATTTTCAGTATG
GGCATCGGCTATACAGATGCCCACTTGCTCGCCTCGATAATGCTTGATCAGCGAGCGGCGTTATGGACCAGGGACAAGCG
TCTGCAGGCGGCGGCCGAAAAGGCAGGCGCTTCATTGCATAAGCCTGCGAATGCTCACAACTAA
GTGATACTGGCAGACACTTCTATATGGATCGATCATTTCCGCCATCTTGATGCAGAGCTTCGGACGATCATTGAGGACGA
TCGTCTACTCTGCCATCCGGCTGTGGCTGGCGAACTGGCGCTTGGCAGCCTTCGAGATCGCGGGCGCGTGATAGCGTTTC
TGGCGGCCCAGCGCCAAGCGTTCGTCGCAACGCACGACGAAGTGATGACAATGATTGATCGTCACGGCATTTTCAGTATG
GGCATCGGCTATACAGATGCCCACTTGCTCGCCTCGATAATGCTTGATCAGCGAGCGGCGTTATGGACCAGGGACAAGCG
TCTGCAGGCGGCGGCCGAAAAGGCAGGCGCTTCATTGCATAAGCCTGCGAATGCTCACAACTAA
Antitoxin
Download Length: 225 bp
>AT185194 NZ_CP066841:c86168-85944 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
