185188

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3894402..3894943 Replicon chromosome
Accession NZ_CP066839
Organism Escherichia coli strain Ec-RL2-1X

Toxin (Protein)


Gene name yafQ Uniprot ID A0A140NAP5
Locus tag JH656_RS19220 Protein ID WP_000615981.1
Coordinates 3894665..3894943 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag JH656_RS19215 Protein ID WP_000729704.1
Coordinates 3894402..3894662 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JH656_RS19195 (3890085) 3890085..3890870 - 786 WP_000207564.1 putative lateral flagellar export/assembly protein LafU -
JH656_RS19200 (3890842) 3890842..3892554 + 1713 Protein_3761 flagellar biosynthesis protein FlhA -
JH656_RS19205 (3892770) 3892770..3893267 - 498 WP_000006260.1 REP-associated tyrosine transposase RayT -
JH656_RS19210 (3893443) 3893443..3894216 - 774 WP_001353765.1 C40 family peptidase -
JH656_RS19215 (3894402) 3894402..3894662 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
JH656_RS19220 (3894665) 3894665..3894943 + 279 WP_000615981.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
JH656_RS19225 (3895099) 3895099..3895839 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
JH656_RS19230 (3895810) 3895810..3896577 - 768 WP_000333379.1 class II glutamine amidotransferase -
JH656_RS19235 (3896783) 3896783..3897361 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA / tssC / AHA_1833 / tssF / tssA / hcp1/tssD1 3887251..3914562 27311


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10860.65 Da        Isoelectric Point: 10.0702

>T185188 WP_000615981.1 NZ_CP066839:3894665-3894943 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQSSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T185188 NZ_CP088094:c140330-140037 [Rhizobium laguerreae]
ATGAGTTTTAAGCTTTCTGCCGAAGCGGAAGAGGACATTATTGCGATCGCCGAGCAAGGTGTCCGTATGTTCGGGGCAGA
CCAAGCAAAGCGATACCACGATGAACTTTTCGCATTATTTGATCTGATAGCTGTCAATCCACGCATCGCGCGCAAACGGG
ATGAGATTGATCCGCCCGTTAGAATTCATCCCTTCAAAGCGCATCTAATCGTCTATCGGATCGAAAATGATGAGACAATT
TTTGTAATACGAATTCGCCACGCGCATGAAGATTGGGCAAGTGATTCAATTTAG

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT185188 WP_000729704.1 NZ_CP066839:3894402-3894662 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT185188 NZ_CP088094:c140569-140327 [Rhizobium laguerreae]
ATGGCCACGATGAACGTATCTCTTCCGGGCCCGATGAAGGATTGGGTGGAGGCCCAGGCCAAGACCGGGCGATATTCGAA
TGCTAGCGACTACGTGCGCGACCTGATCCGACGCGACCAAACGCGAAACGACAAGATCGCCGCCATGCAAAGCTTCGTCG
AGGCCGGGCTCCAAAGCGGTGTCGGCGTCCGATCAAAGGATGAACTCTTTGCTGAGGCGATGGCTCGTGCCAGCAAGTCA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MMG
PDB 4MMJ
PDB 4ML2
PDB 4ML0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References